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Updated: May 14, 2026

Agarose Fluid Gels Formed by Shear Processing During Gelation for Suspended 3D Bioprinting
Published on: May 26, 2023
Biofabrication in suspension media-a decade of advances
Megan E Cooke1, Morgan B Riffe2, Manuela E Gomes3
1BioFrontiers Institute, University of Colorado Boulder, Boulder CO 80303, United States of America.
Abstract:
Suspension bath bioprinting, defined as extrusion bioprinting into a suspension bath consisting of a yield-stress material with fast recovery, emerged over a decade ago. Since this time, many suspension baths have been developed from molecular assemblies to granular media and across a range of synthetic and natural polymers. These suspension baths have been applied to the printing of a wide variety of inks for applications in tissue engineering, fromin vitrotissue models to implantable constructs. In a scoping search of published literature over the past decade, 254 articles were identified that met various definitions related to suspension baths for biofabrication in order to gain a perspective on the various materials used and their applications; however, the literature is much more broad than this due to the disperse terminology that has been applied to the approach. This article gives a perspective on the progress that has been made in suspension bath printing, including applications of the technology and challenges that exist across the field, as well as provides a look to the future of where such printing methods will make an impact.

