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Linking Epitope-Specific T-Cell Receptors to IFNγ Secretion Using Nanovial Technology
Jet van den Dijssel1,2,3, Tejas Menon3, Hayley A McQuilten3
1Sanquin Research and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands.
To link IFNγ-secretion levels of epitope-specific T-cells with their TCRαβ, we coated nanovials with pHLA-I to capture and activate epitope-specific T-cells and their secreted IFNγ, followed by index-sorting and TCRαβ sequencing. We demonstrate that nanovials are a promising tool to link single epitope-specific TCRαβ clonotypes to the cell's functional properties.
To link IFNγ-secretion levels of epitope-specific T-cells with their TCRαβ, we coated nanovials with pHLA-I to capture and activate epitope-specific T-cells and their secreted IFNγ, followed by index-sorting and TCRαβ sequencing. We demonstrate that nanovials are a promising tool to link single epitope-specific TCRαβ clonotypes to the cell's functional properties.
