Tuning the binding process of rapeseed protein with quercetin for stabilizing high internal phase emulsions

Regulation in protein-polyphenols interaction contributes to their application in emulsion, but relative information is very limited. Therefore, this work designed a novel strategy to regulate the interaction of rapeseed protein (RP) with quercetin (Que) for stabilizing high internal phase emulsions (HIPEs) loaded with vitamin D3 (V_D3). The results showed that whey protein enhanced the interaction of RP with water molecular, and zein contributed to its binding with Que. Modified RP-Que complex possessed higher Que-binding rate (76.15 % vs 96.86 %), DPPH· scavenging rate (45.33 % vs 74.89 %), surface charge (-24 mV), orderliness as well as smaller size (200 nm). Above improvements increased its diffusion rate (0.74 mN/m/s^1/2), modulus and adsorption content (350 %) on the oil-water interface, which formed denser network structure in HIPEs, and gave them higher bioavailability and stability of V_D3 and storage stability of 3D-printing products. Overall, molecular regulation improved the application potential of RP-Que complex in HIPEs.