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Updated: Aug 23, 2026

A Method for Targeted 16S Sequencing of Human Milk Samples
Published on: March 23, 2018
Pathogenic characterization and mechanism of sequence type 4 Cronobacter sakazakii derived from milk-based infant and
Yuanyuan Zhang1,2,3, Bin Zhang3, Yunjiao Han3
1Hebei Normal University, Hebei, 050010, China.
Background:
Cronobacter sakazakii can cause severe infections in premature infants and neonates through the consumption of contaminated milk-based foods. However, the pathogenesis of sequence type 4 (ST4) C. sakazakii remains to be fully elucidated.
Results:
In this study, four ST4 C. sakazakii strains were investigated via comparative toxicity, genomic, and transcriptomic analyses to elucidate their pathogenic characteristics and mechanisms. Multilocus sequence typing (MLST) indicated that ST4 C. sakazakii was frequently identified among 36 Cronobacter spp. isolates recovered from milk-based infant and baby foods, and 13 novel STs were also detected. Compared with other ST isolates, ST4 C. sakazakii displayed a higher gut weight to carcass weight ratio (GW/CW), stronger abilities to invade and translocate, and increased secretion of TNF-α, IL-1, and lactate dehydrogenase (LDH) in human brain microvascular endothelial cells (HBMECs) and human U251 glioma cells (U251). Moreover, ST4 C. sakazakii strains with a higher GW/CW ratio significantly disrupted routine blood indices, promoted the secretion of inflammatory factors, and induced severe histopathological changes in the liver, brain, spleen, kidney, and intestine of suckling mice. Although differences in genome composition and known virulence factors were observed among these ST4 C. sakazakii strains with varying pathogenic phenotypes, comparative transcriptomic analyses revealed that the expression of numerous virulence factors and pathways, including ompA, ompW, luxS, rpoS, the Sec secretion system, lipopolysaccharide biosynthesis and assembly, and flagellar assembly, greatly contributed to the high pathogenicity of ST4 C. sakazakii.
Conclusion:
Our findings suggest that foodborne ST4 C. sakazakii isolates represent a significant potential threat to food safety and public health, particularly for premature and immunocompromised infants.
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