Related Experiment Video

Updated: Sep 21, 2026

In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells
09:20

In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells

Published on: July 23, 2010

An association between replicating adenovirus DNA and the nuclear matrix of infected HeLa cells

H B Younghusband

    Canadian Journal of Biochemistry and Cell Biology = Revue Canadienne De Biochimie Et Biologie Cellulaire
    |June 1, 1985
    PubMed

    Insights

    Newly synthesized human adenovirus type 5 DNA associates with the nuclear matrix in infected HeLa cells. This viral DNA remains matrix-bound and nuclease-resistant throughout its lifecycle.

    Area of Science:

    • Molecular Biology
    • Virology
    • Cell Biology

    Background:

    • Human adenovirus type 5 (HAdV5) is a significant human pathogen.
    • Understanding viral DNA replication and its cellular localization is crucial for antiviral strategies.

    Purpose of the Study:

    • To investigate the association of newly synthesized HAdV5 DNA with the nuclear matrix.
    • To determine the nuclease sensitivity of viral DNA during replication.

    Main Methods:

    • HeLa cells were infected with HAdV5 and pulse-labeled with [3H]thymidine.
    • Nuclear matrices were isolated from infected cells.
    • DNase I digestion was used to assess DNA-protein interactions and localization.

    Main Results:

    • Over 95% of newly synthesized viral DNA was associated with the nuclear matrix after a 1-min pulse.
    • Viral DNA demonstrated resistance to DNase I digestion compared to bulk cellular DNA.
    • This matrix association and nuclease resistance persisted even after longer pulses or chase periods.

    Conclusions:

    • Viral DNA synthesis occurs in association with the nuclear matrix.
    • Newly synthesized viral DNA remains associated with the nuclear matrix until its incorporation into progeny virus particles.
    • This association may be mediated by viral proteins or the matrix structure itself, conferring nuclease resistance.

    More Related Videos

    Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells10:22

    Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells

    Published on: November 12, 2015

    Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry09:38

    Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry

    Published on: June 26, 2019

    Related Concept Videos

    What are Viruses?00:50

    What are Viruses?

    Overview
    Retrovirus Life Cycles01:10

    Retrovirus Life Cycles

    Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the retrovirus to...
    Viral Recombination00:57

    Viral Recombination

    Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
    Mechanisms of Retrovirus-induced Cancers01:51

    Mechanisms of Retrovirus-induced Cancers

    Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
    Rous Sarcoma Virus (RSV) and Cancer01:03

    Rous Sarcoma Virus (RSV) and Cancer

    Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
    RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
    Size and Structure of Viral Genomes01:26

    Size and Structure of Viral Genomes

    Viral genomes exhibit remarkable diversity in size, structure, and composition, influencing their replication strategies and interactions with host cells. These genomes consist of either DNA or RNA and may be linear or circular. Additionally, they can be single-stranded or double-stranded, with each configuration affecting how the virus propagates within a host. RNA viruses, for instance, generally have smaller genomes than DNA viruses, a factor that contributes to their high mutation rates and...