Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Imaging Studies III: Computed Tomography

Imaging Studies III: Computed Tomography

DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...

Imaging Studies V: Intravenous Urography and Retrograde Pyelography

Imaging Studies V: Intravenous Urography and Retrograde Pyelography

IntroductionIntravenous Urography (IVU) and Retrograde Pyelography (RP) are important diagnostic imaging techniques used to evaluate the urinary system. These methods help identify structural abnormalities, obstructions, and functional issues in the kidneys, ureters, and bladder. Both procedures use iodine-based contrast media to enhance the visibility of urinary tract structures on X-ray images, though they differ in their methods and indications.1. Intravenous Urography (IVU)Intravenous...

Drug Dosing in Renal Diseases: Measurement of Glomerular Filtration Rate

Drug Dosing in Renal Diseases: Measurement of Glomerular Filtration Rate

The glomerular filtration rate (GFR) is a critical indicator of kidney health, reflecting how well the kidneys filter blood. Changes in GFR can signal potential kidney impairment, necessitating accurate measurement methods to monitor kidney function effectively.Various molecules can serve as markers for GFR measurement, with the ideal marker meeting several specific criteria. It must freely filter at the glomerulus, avoid reabsorption or secretion by the renal tubules, remain unmetabolized, not...

Imaging Studies IV: Magnetic Resonance Imaging

Imaging Studies IV: Magnetic Resonance Imaging

Introduction:Magnetic Resonance Imaging, or MRI, can include a specialized imaging technique of the urinary system known as Magnetic Resonance Urography (MRU). This radiation-free technique uses strong magnetic fields and radio waves to produce detailed images with the help of a computer. MRU is particularly effective for visualizing fluid-filled structures like the kidneys, ureters, and bladder.Applications of MRI in the Genitourinary SystemKidneys and Ureters: MRI detects tumors, cysts,...

Kidney Transplant II: Surgical Procedure

Kidney Transplant II: Surgical Procedure

Preoperative ManagementThe primary goals of preoperative management in kidney transplantation are to optimize the patient’s metabolic state and prepare them for surgery through diet adjustments, necessary dialysis, and tailored medical treatment. This phase also involves comprehensive infection screening and patient education about the surgical procedure and postoperative care to improve outcomes and adherence.Medical ManagementA comprehensive evaluation is required for both the living donor...

Nursing Assessment of the Genitourinary System II: Inspection and Palpation

Nursing Assessment of the Genitourinary System II: Inspection and Palpation

The nursing assessment of the genitourinary (GU) system involves a systematic inspection and palpation to identify abnormalities in the kidneys, bladder, and surrounding structures.InspectionMouth: Inspect for signs of kidney dysfunction, such as stomatitis (inflammation of the mouth) and ammonia breath, which may occur in advanced kidney disease due to the buildup of urea, breaking down into ammonia.Skin: Check for pallor, which could indicate anemia caused by kidney disease. Look for...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Introduction of new calibrator lots for quantitative protein mass spectrometry and adjustment of calibration settings for long term multiplex apolipoproteins quantification.

Clinical chemistry and laboratory medicine·2026

Same author

C5b-9 Deposition in Renal Transplant Biopsies Associates With Prolonged Delayed Graft Function in Kidneys Donated After Circulatory Death.

Transplantation·2026

Same author

Correlation and Commutability Study between an LC-MS-Based Reference Measurement Procedure and Immunoassays Measuring Apolipoprotein A-I and Apolipoprotein B.

Clinical chemistry·2026

Same author

Small, dense LDL-C as a predictor of cardiovascular risk and benefit of alirocumab in patients with recent acute coronary syndrome receiving optimized statin treatment.

American journal of preventive cardiology·2026

Same author

Analytical validation of an IFCC-endorsed mass spectrometry-based semi-automated reference measurement procedure for global standardization of serum apolipoprotein(a).

Clinical chemistry and laboratory medicine·2026

Same author

Development and Analytical Validation of a Multiplex LC-MRM-MS-Based Reference Measurement Procedure for Apolipoprotein A-I and Btotal Quantification in Serum.

Clinical chemistry·2026

Search research articles

Related Experiment Video

Updated: May 10, 2026

Non-invasive Imaging of Acute Allograft Rejection after Rat Renal Transplantation Using 18F-FDG PET

Non-invasive Imaging of Acute Allograft Rejection after Rat Renal Transplantation Using ¹⁸F-FDG PET

Published on: April 28, 2013

Urinary NGAL Outperforms ^99mTc-MAG3 Renography in Predicting DCD Kidney Graft Function.

Esther N M de Rooij^1,2, Tirsa T van Duijl³, Ellen K Hoogeveen^1,2,4

¹Department of Nephrology, Leiden University Medical Center, Leiden, Netherlands.

Transplant International : Official Journal of the European Society for Organ Transplantation

|May 27, 2025

Summary

Urinary neutrophil gelatinase-associated lipocalin (NGAL) and its fractional excretion (FE-NGAL) can predict delayed graft function (DGF) in kidney transplant recipients. These biomarkers show promise as alternatives to traditional renography for monitoring DGF.

Keywords:

DCD donation NGAL delayed graft function (DGF)kidney transplantation urinary biomarkers

More Related Videos

Point-of-Care Kidney and Genitourinary Ultrasound in Adults: Image Acquisition

Point-of-Care Kidney and Genitourinary Ultrasound in Adults: Image Acquisition

Published on: June 21, 2024

An Immature Murine Model of Reversible Unilateral Ureteral Obstruction

An Immature Murine Model of Reversible Unilateral Ureteral Obstruction

Published on: April 4, 2025

Related Experiment Videos

Last Updated: May 10, 2026

Non-invasive Imaging of Acute Allograft Rejection after Rat Renal Transplantation Using 18F-FDG PET

Non-invasive Imaging of Acute Allograft Rejection after Rat Renal Transplantation Using ¹⁸F-FDG PET

Published on: April 28, 2013

Point-of-Care Kidney and Genitourinary Ultrasound in Adults: Image Acquisition

Point-of-Care Kidney and Genitourinary Ultrasound in Adults: Image Acquisition

Published on: June 21, 2024

An Immature Murine Model of Reversible Unilateral Ureteral Obstruction

An Immature Murine Model of Reversible Unilateral Ureteral Obstruction

Published on: April 4, 2025

Area of Science:

Nephrology and Transplant Surgery
Biomarker Discovery and Validation
Ischemia-Reperfusion Injury Research

Background:

Donation after circulatory death (DCD) kidney recipients face high risks of delayed graft function (DGF) due to ischemia-reperfusion injury.
Accurate prediction and monitoring of DGF are crucial for optimizing transplant outcomes.
Current monitoring methods like tubular function slope (TFS) using 99mTc-MAG3 renography have limitations.

Purpose of the Study:

To compare the predictive performance of urinary biomarkers for DGF duration against the gold standard TFS in DCD kidney transplant recipients.
To evaluate novel urinary biomarkers, including NGAL and FE-NGAL, for their ability to predict DGF severity and progression.

Main Methods:

Quantification of urinary TIMP-2, IGFBP7, B2M, NGAL, KIM1, CXCL9, and UMOD via LC-MS/MS in 89 DCD kidney transplant recipients at postoperative days (PODs) 1, 4, and 10.
Assessment of interstitial fibrosis and tubular atrophy (IF/TA) using protocol biopsies at POD 10.
Calculation of TFS using 99mTc-MAG3 renography and comparison of predictive performance using ROC analyses.

Main Results:

Urinary NGAL and FE-NGAL at POD 4 demonstrated superior predictive performance for DGF compared to TFS and other biomarkers (AUCs 0.97 and 0.98, respectively).
At POD 10, FE-NGAL and PCR best predicted severe versus mild to moderate DGF (AUCs 0.74 and 0.76), outperforming TFS (AUC 0.65).
A significant association was found between IF/TA and DGF severity, with higher odds for severe DGF (OR 15.0).

Conclusions:

Urinary NGAL and FE-NGAL are promising non-invasive biomarkers for predicting and monitoring DGF in DCD kidney transplant recipients.
These biomarkers may serve as a viable alternative to 99mTc-MAG3 renography for assessing DGF clearance.
NGAL and FE-NGAL could guide decisions for kidney transplant biopsies, potentially excluding additional acute rejection.