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Published on: March 20, 2015
Enhanced abscisic acid detection via SERS-active single crystal MAPbCl3 nanofiber-based Self-CoAptaNano (SCAN)
Muhammad Awais1, Syed Muhammad Zaigham Abbas Naqvi1, Yanyan Zhang1
1College of Mechanical and Electrical Engineering, Henan Agricultural University, Zhengzhou, 450002, China; Henan International Joint Laboratory of Laser Technology in Agriculture Sciences, Zhengzhou, 450002, China; State Key Laboratory of Wheat and Maize Crap Science, Zhengzhou, 450002, China.
A new Self-CoAptaNano (SCAN) substrate using MAPbCl3 nanofibers enables sensitive, label-free detection of the plant hormone abscisic acid (ABA). This SERS-active substrate offers improved detection limits and recovery rates for ABA analysis in plant samples.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Materials Science
Background:
- Accurate detection of plant stress phytohormone abscisic acid (ABA) is crucial for understanding plant physiology.
- Conventional methods for ABA detection often lack sensitivity, speed, or require labeling.
- Aptamers offer specific molecular recognition capabilities for targeted analyte binding.
Purpose of the Study:
- To develop a novel, sensitive, and label-free substrate for abscisic acid (ABA) detection.
- To utilize SERS-active MAPbCl3 nanofibers functionalized with aptamers for enhanced ABA detection.
- To investigate the thermodynamic stability and targeting specificity of the designed aptamer sequence.
Main Methods:
- Fabrication of SERS-active MAPbCl3 nanofibers.
- Design and bioinformatic analysis of aptamers targeting the LOC109791758 gene (encoding GRCWP).
- Characterization of the Self-CoAptaNano (SCAN) substrate using SEM, UV-Visible spectroscopy, and SERS.
- Evaluation of ABA detection performance including limit of detection (LOD), enhancement factor (EF), recovery rate, and relative standard deviation (RSD).
Main Results:
- The developed MAPbCl3 nanofiber-based SCAN substrate demonstrated a low LOD of 1.17 × 10⁻¹² M for SERS.
- An enhancement factor (EF) of 1.08 × 10⁷ M was achieved with high recovery rates (≈100%) and low RSD (3.24% for SERS).
- The substrate showed effective ABA detection in complex plant matrices, outperforming previous methods.
Conclusions:
- The novel SERS-active MAPbCl3 nanofiber-based SCAN substrate provides a rapid, sensitive, and label-free platform for ABA detection.
- The aptamer's stable configuration and specific targeting enhance the substrate's performance.
- This technology holds significant potential for broader applications in analytical chemistry and biotechnology for plant analysis.
