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Exploring the Targets of Reactive Oxygen Species and Defense against Oxidative Stress in Campylobacter jejuni Using a

Nova Mondry Cohen1,2, Chiranth Krishna Kumar1,2, Haruta Iitoyo1,2

  • 1School of Life and Environmental Sciences, The University of Sydney , Sydney2006, Australia.

Journal of Proteome Research
|May 28, 2025
PubMed

Insights

Campylobacter jejuni survival relies on managing reactive oxygen species (ROS). This study reveals how ROS impacts C. jejuni proteins and metabolism, identifying potential targets for new antimicrobials.

Area of Science:

  • Microbiology
  • Proteomics
  • Biochemistry

Background:

  • Campylobacter jejuni causes human gastroenteritis, necessitating understanding its pathogenesis.
  • Bacterial survival against reactive oxygen species (ROS) is crucial for infection.

Purpose of the Study:

  • To investigate the proteomic and metabolic responses of C. jejuni to oxidative stress.
  • To identify key proteins and pathways involved in ROS resistance and potential antimicrobial targets.

Main Methods:

  • Label-based proteomics using LC-MS/MS to quantify protein abundance changes under hydrogen peroxide and paraquat stress.
  • Analysis of nutrient transporter and outer membrane protein expression.
  • Identification of cysteine oxidation modifications (sulfinic/sulfonic acids) using SCX and HILIC coupled LC-MS/MS.

Main Results:

  • Antioxidant proteins (KatA, AhpC) were induced, confirming oxidative stress response.
  • ROS altered nutrient transporter levels and outer membrane protein Cj1170c abundance, impacting survival and metabolite levels.
  • Extensive cysteine oxidation was observed, with iron-sulfur clusters being primary ROS targets.
  • Evidence suggests an endogenous oxidative intracellular environment in C. jejuni.

Conclusions:

  • C. jejuni employs specific protein and metabolic adaptations to counteract ROS.
  • The outer membrane protein Cj1170c and cysteine oxidation sites are critical for survival.
  • Understanding these redox adaptations offers opportunities for developing novel antimicrobial strategies.
Keywords:
bacterial pathogenesiscysteinemetabolomicsnutrient transportpost-translational modificationssulfinic/sulfonic acid

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