Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Nucleolus02:55

The Nucleolus

10.6K
The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
10.6K
The Nucleolus02:55

The Nucleolus

6.4K
6.4K
Additional Subnuclear Structures02:10

Additional Subnuclear Structures

5.5K
The eukaryotic nucleus is a double membrane-bound organelle that contains nearly all of the cell’s genetic material in the form of chromosomes. It is rightly called the “brain” of the cell as it shoulders the responsibility of responding to various physiological processes, stress, altered metabolic conditions, and other cellular signals. 
The nucleus contains many membrane-less subnuclear organelles or nuclear bodies, such as nucleoli, Cajal bodies, speckles,...
5.5K
Nucleosome Remodeling02:54

Nucleosome Remodeling

11.6K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
11.6K
Nucleoid01:24

Nucleoid

1.6K
The nucleoid represents a structurally and functionally distinct region within prokaryotic cells, where the cell's DNA and associated proteins are housed. Unlike eukaryotic cells, prokaryotes lack a membrane-bound nucleus, and the nucleoid facilitates the organization and accessibility of the genetic material within this constraint. The DNA in most bacteria and archaea exists as a single, circular, double-stranded molecule that is highly compacted through supercoiling and interactions with...
1.6K
The Nucleus01:25

The Nucleus

8.3K
The nucleus is a membrane-bound organelle that acts as a control center in a eukaryotic cell. It contains chromosomal DNA, which controls gene expression and precisely regulates the production of proteins within the cell. In contrast, the DNA inside the mitochondria and chloroplast only carries out functions that are specific to those organelles.
Arrangement of DNA within Nucleus
The regulation of gene expression inside the nucleus is dependent on many factors, including the DNA structure. The...
8.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ClC-7 Deficiency Impairs Tooth Development and Eruption.

Scientific reports·2016
Same author

The Complete Genome of Brucella Suis 019 Provides Insights on Cross-Species Infection.

Genes·2016
Same author

An adaptive immune response driven by mature, antigen-experienced T and B cells within the microenvironment of oral squamous cell carcinoma.

International journal of cancer·2016
Same author

Music exposure improves spatial cognition by enhancing the BDNF level of dorsal hippocampal subregions in the developing rats.

Brain research bulletin·2016
Same author

Impact of perioperative blood transfusion on immune function and prognosis in colorectal cancer patients.

Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis·2016
Same author

Ultrathin Co3O4 Layers Realizing Optimized CO2 Electroreduction to Formate.

Angewandte Chemie (International ed. in English)·2016

Related Experiment Video

Updated: Mar 22, 2026

A Cell Free Assay to Study Chromatin Decondensation at the End of Mitosis
11:04

A Cell Free Assay to Study Chromatin Decondensation at the End of Mitosis

Published on: December 19, 2015

10.9K

FuHsi maintains nucleolar integrity.

Xiaohui Yang1, Ziyue Jiang2, Songzhe Wu2

  • 1School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230000, China; Key Laboratory of Biomedical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, 215163, China.

Biochemical and Biophysical Research Communications
|May 28, 2025
PubMed
Summary

FuHsi is crucial for nucleolar architecture, anchoring ribosomal DNA (rDNA) and initiating nucleolus formation. Its depletion disperses rDNA, revealing its upstream role in the nucleolar assembly cascade.

More Related Videos

Heterokaryon Technique for Analysis of Cell Type-specific Localization
09:31

Heterokaryon Technique for Analysis of Cell Type-specific Localization

Published on: March 11, 2011

17.1K
Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry
09:38

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry

Published on: June 26, 2019

8.7K

Related Experiment Videos

Last Updated: Mar 22, 2026

A Cell Free Assay to Study Chromatin Decondensation at the End of Mitosis
11:04

A Cell Free Assay to Study Chromatin Decondensation at the End of Mitosis

Published on: December 19, 2015

10.9K
Heterokaryon Technique for Analysis of Cell Type-specific Localization
09:31

Heterokaryon Technique for Analysis of Cell Type-specific Localization

Published on: March 11, 2011

17.1K
Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry
09:38

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry

Published on: June 26, 2019

8.7K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • The nucleolus, a key organelle for ribosome biogenesis, is a dynamic structure.
  • Nucleolar structural organization is intrinsically linked to its function.
  • FuHsi was previously identified as a novel ribosomal DNA (rDNA) transcriptional regulator.

Purpose of the Study:

  • To elucidate the role of FuHsi in nucleolar architecture.
  • To understand FuHsi's position within the nucleolar assembly pathway.
  • To investigate the impact of FuHsi on ribosomal DNA organization.

Main Methods:

  • Immunofluorescence microscopy to assess co-localization of proteins and rDNA.
  • RNA interference (RNAi) to knock down FuHsi, TCOF1, UBF, and RPA194.
  • Analysis of nucleolar component distribution following knockdown experiments.

Main Results:

  • FuHsi co-localizes with UBF, RPA194, TCOF1, and rDNA within the nucleolus.
  • FuHsi knockdown causes dispersal of rDNA and loss of other nucleolar components.
  • FuHsi depletion results in a unique phenotype not observed with TCOF1 knockdown.
  • TCOF1 knockdown displaces UBF but not FuHsi or rDNA.
  • UBF or RPA194 knockdown causes peripheral redistribution of FuHsi.

Conclusions:

  • FuHsi functions upstream of TCOF1 and UBF in nucleolar assembly.
  • FuHsi is essential for anchoring rDNA at the nucleolar core.
  • FuHsi plays a novel, initiating role in rDNA organization and nucleolar formation.