Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Operative management of pediatric radiation-induced cavernous malformations: insights from a single-center cohort.

Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery·2026
Same author

Structure-Permeability relationships of Vitamin-B-Based quaternary ammonium salts for oral drug development.

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V·2026
Same author

Sustainable depletion of high-abundance serum proteins using aqueous biphasic systems based on glycine-betaine analogue ionic liquids for the detection of masked biomarkers.

Journal of chromatography. A·2026
Same author

Pediatric sinogenic and otogenic intracranial infections requiring neurosurgical intervention: a North American multicenter study in the era of COVID-19.

Journal of neurosurgery. Pediatrics·2026
Same author

Controlling serum protein depletion in aqueous biphasic systems: interphase precipitation versus bottom phase retention for bisphenol A monitoring.

Analytica chimica acta·2026
Same author

Management of pediatric patients with moyamoya arteriopathy and middle aortic syndrome: a retrospective single-institution case series.

Journal of neurosurgery. Pediatrics·2026

Related Experiment Video

Updated: Sep 20, 2025

Author Spotlight: Enhancing Lipid Nanoparticle Formation Through Turbulent Mixing in Confined Geometries
08:10

Author Spotlight: Enhancing Lipid Nanoparticle Formation Through Turbulent Mixing in Confined Geometries

Published on: August 23, 2024

4.5K

Mitigating Shear Stress in Spray Drying for RNA-Loaded Lipid Nanoparticles through Process and Formulation

Susana Farinha1,2,3, Rute Mota1,2,4,5, Carolina Lopes1

  • 1R&D Inhalation and Advanced Drug Delivery, Hovione Farmaciência S.A, Estrada Do Lumiar, Campus Do Lumiar, Edifício R, Lisbon, 1649-038, Portugal.

AAPS Pharmscitech
|May 28, 2025
PubMed
Summary

Spray drying enhances the stability of lipid nanoparticles (LNPs) for nucleic acid therapies. Optimizing spray drying with Poloxamer 188 protects RNA-loaded LNPs, overcoming cold chain limitations.

Keywords:
RNAfreeze dryinglipid nanoparticlesprocess and formulation developmentspray drying

More Related Videos

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform
09:41

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Published on: February 25, 2021

23.7K
Testing the In Vitro and In Vivo Efficiency of mRNA-Lipid Nanoparticles Formulated by Microfluidic Mixing
08:55

Testing the In Vitro and In Vivo Efficiency of mRNA-Lipid Nanoparticles Formulated by Microfluidic Mixing

Published on: January 20, 2023

12.1K

Related Experiment Videos

Last Updated: Sep 20, 2025

Author Spotlight: Enhancing Lipid Nanoparticle Formation Through Turbulent Mixing in Confined Geometries
08:10

Author Spotlight: Enhancing Lipid Nanoparticle Formation Through Turbulent Mixing in Confined Geometries

Published on: August 23, 2024

4.5K
Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform
09:41

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Published on: February 25, 2021

23.7K
Testing the In Vitro and In Vivo Efficiency of mRNA-Lipid Nanoparticles Formulated by Microfluidic Mixing
08:55

Testing the In Vitro and In Vivo Efficiency of mRNA-Lipid Nanoparticles Formulated by Microfluidic Mixing

Published on: January 20, 2023

12.1K

Area of Science:

  • Biotechnology
  • Materials Science
  • Pharmaceutical Sciences

Background:

  • Lipid nanoparticles (LNPs) are crucial for nucleic acid therapies, including mRNA vaccines.
  • Current LNP therapies face stability challenges, necessitating cold chain storage.
  • Improving LNP stability is vital for broader therapeutic accessibility.

Purpose of the Study:

  • To optimize spray drying (SD) for enhanced stability of ribonucleic acid (RNA)-loaded LNPs.
  • To investigate formulation and process parameters for maintaining LNP integrity post-drying.
  • To evaluate the protective role of Poloxamer 188 (P188) during spray drying.

Main Methods:

  • Microfluidics used for high-efficiency encapsulation (>95%) of RNA into LNPs.
  • Systematic optimization of spray drying (SD) process and formulation.
  • Benchmarking SD against freeze drying (FD) for LNP stability.
  • Analysis of colloidal stability and RNA encapsulation efficiency post-drying.

Main Results:

  • Optimized SD process successfully maintained LNP colloidal stability and RNA encapsulation.
  • Poloxamer 188 (P188) demonstrated significant protective effects against shear stress during SD.
  • Spray drying proved effective in enhancing LNP stability compared to traditional methods.
  • Microfluidic production yielded highly efficient RNA encapsulation in LNPs.

Conclusions:

  • Spray drying is a viable technology for stabilizing RNA-loaded LNPs.
  • Formulation and process optimization, particularly with P188, are key to successful SD of LNPs.
  • Enhanced LNP stability through drying technologies can reduce reliance on cold chains.
  • This study highlights potential for improved delivery and accessibility of nucleic acid therapies.