Related Experiment Video

Updated: Jan 18, 2026

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
10:31

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

618

Characterizing metabolic dysregulation in early-stage chronic kidney disease for diagnostic insights

Upasna Gupta1,2, Amrita Sahu1,2, Dharmendra Singh Bhadauria3

  • 1Centre of Biomedical Research, Sanjay Gandhi Post Institute of Medical Sciences Campus, Raebareli Road, Lucknow, Uttar Pradesh, 226014, India. neerajcbmr@gmail.com.

Molecular Omics
|May 29, 2025
PubMed

Insights

Early diagnosis of chronic kidney disease (CKD) is difficult. This study identified ten key metabolites in serum using metabolomics, offering potential biomarkers for early CKD detection and personalized treatment strategies.

Area of Science:

  • * Biochemistry and metabolomics
  • * Renal medicine and diagnostics

Background:

  • * Chronic kidney disease (CKD) presents diagnostic challenges in early stages (G1-G3) using conventional methods like serum creatinine.
  • * Early detection and identification of biomarkers for personalized treatment in early CKD remain underexplored.
  • * Glomerular filtration rate (GFR) ≥30 mL/min/1.73 m² defines early-stage CKD, indicating normal to moderate kidney function.

Purpose of the Study:

  • * To identify novel biomarkers for early detection of chronic kidney disease (CKD).
  • * To investigate physiological changes associated with early-stage CKD.
  • * To explore potential for personalized treatment strategies in early CKD management.

Main Methods:

  • * A metabolomic analysis utilizing proton nuclear magnetic resonance (1H NMR) on 115 human serum samples (24 healthy controls, 91 early-stage CKD patients).
  • * Data pre-processing and statistical analyses performed using MetaboAnalyst 6.0, including PCA, PLS-DA, OPLS-DA, ANOVA, and Wilcoxon Mann-Whitney test.
  • * Random forest modeling for CKD stage differentiation, KEGG database for pathway enrichment, and ROC analysis for metabolite diagnostic value assessment.

Main Results:

  • * Ten metabolites (myo-Inositol, glycerol, pyruvate, carnitine, phenylalanine, tyrosine, histidine, TMAO, 2-hydroxyisobutyrate, 3-hydroxyisobutyrate) showed significant changes across CKD stages (p < 0.05, VIP > 1).
  • * Receiver operating characteristic (ROC) curve analysis indicated diagnostic potential with AUC values > 0.7 for identified metabolites.
  • * Pathway analysis revealed significant dysregulation in inositol phosphate, tyrosine, histidine, pyruvate metabolism, and phenylalanine, tryptophan, and tyrosine biosynthesis.

Conclusions:

  • * This metabolomics study successfully identified potential biomarkers for early-stage chronic kidney disease (CKD).
  • * Significant metabolic abnormalities and pathway dysregulations were observed in early CKD patients.
  • * The findings support the development of individualized care strategies for managing early CKD.

More Related Videos

5/6 Nephrectomy Using Sharp Bipolectomy Via Midline Laparotomy in Rats05:34

5/6 Nephrectomy Using Sharp Bipolectomy Via Midline Laparotomy in Rats

Published on: April 4, 2025

1.5K
Use of Ultra-high Field MRI in Small Rodent Models of Polycystic Kidney Disease for In Vivo Phenotyping and Drug Monitoring07:35

Use of Ultra-high Field MRI in Small Rodent Models of Polycystic Kidney Disease for In Vivo Phenotyping and Drug Monitoring

Published on: June 23, 2015

11.9K

Related Concept Videos

Chronic Kidney Disease I: Introduction01:25

Chronic Kidney Disease I: Introduction

Chronic Kidney Disease (CKD) arises when the kidneys progressively lose their ability to function, ultimately leading to end-stage renal disease. At this advanced stage, the kidneys can no longer filter waste or maintain essential body functions, requiring renal replacement therapy (RRT) through dialysis or a kidney transplant for survival.Early-stage chronic kidney disease and detection challengesIn CKD's early stages, symptoms often remain absent because healthy nephrons compensate for...
573
Chronic Kidney Disease III: Interprofessional Care01:28

Chronic Kidney Disease III: Interprofessional Care

Chronic kidney disease (CKD) requires collaborative and comprehensive management. CKD progresses through stages and can lead to end-stage kidney disease (ESKD) if untreated. Interprofessional collaboration and patient education are crucial, enabling patients to manage their health and improve their quality of life.Diagnostic approach for chronic kidney diseaseThe diagnosis of CKD primarily focuses on the glomerular filtration rate (GFR), which assesses kidney function by measuring how well...
346
Chronic Kidney Disease II: Clinical Manifestations01:24

Chronic Kidney Disease II: Clinical Manifestations

Chronic Kidney Disease (CKD) progressively impairs multiple body systems due to the accumulation of uremic toxins, which disrupt cellular functions across various organs.Neurologic symptomsNeurologic symptoms often arise early in CKD, as uremic toxin buildup drives changes in cognitive and motor functions. Patients frequently experience fatigue, headache, confusion, difficulty concentrating, and, in severe cases, seizures. Peripheral neuropathy commonly manifests as burning sensations in the...
559
Chronic Kidney Disease IV: Nursing Management01:18

Chronic Kidney Disease IV: Nursing Management

Nursing management is essential for preventing complications, maintaining stability, and improving patients' quality of life in chronic kidney disease (CKD). By using a structured approach, nurses help slow CKD progression and support effective patient care​.1. Comprehensive patient assessmentEffective management begins with nurses reviewing the patient’s medical history, and identifying key risk factors like diabetes, hypertension, and nephrotoxic drug use. Nurses assess signs of...
322
Acute Kidney Injury IV: Diagnostic Studies and Prevention01:30

Acute Kidney Injury IV: Diagnostic Studies and Prevention

Accurate diagnosis and effective prevention are critical in managing Acute Kidney Injury (AKI), which is linked to high mortality rates ranging from 10% to 80%. Timely recognition of at-risk patients and careful monitoring can significantly reduce the likelihood of kidney damage.Diagnostic Assessments:The diagnostic process starts with a comprehensive medical history to identify prerenal, intrarenal, and postrenal causes.Prerenal causes, such as dehydration, hypotension, or blood loss, should...
271
Drug Dosing in Renal Diseases: Estimation of Glomerular Filtration Rate Based on Serum Creatinine Concentration01:28

Drug Dosing in Renal Diseases: Estimation of Glomerular Filtration Rate Based on Serum Creatinine Concentration

Glomerular filtration rate (GFR) can be estimated from serum creatinine using the modification of diet in renal disease (MDRD) formula or the chronic kidney disease–epidemiology collaboration (CKD–EPI) equation. Both methods are widely used in clinical practice to assess kidney function and guide treatment decisions.The MDRD equation does not require weight or height measurements and is normalized to the body surface area of 1.73 m², considered the average adult surface area.
191