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Updated: Sep 20, 2025

Conducting Maximal and Submaximal Endurance Exercise Testing to Measure Physiological and Biological Responses to Acute Exercise in Humans
Published on: October 17, 2018
Performance and Physiologic Responses to Repeated 3-Minute All-Out Shuttle Tests
Benjamin J Mendelson1, Kyle T Ebersole, Scott D Brau
1Human Performance and Sport Physiology Laboratory, School of Rehabilitation Sciences and Technology, College of Health Professions and Sciences, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin.
Repeated 3-minute all-out shuttle tests showed that while critical velocity remained stable, anaerobic work capacity and maximal sprint speed significantly decreased. Maximal aerobic capacity correlated with critical velocity, suggesting it
Area of Science:
- Exercise Physiology
- Sports Science
- Performance Analysis
Background:
- Understanding performance decrements in repeated high-intensity efforts is crucial for training.
- Previous research has not fully elucidated the physiological responses to repeated 3-minute all-out shuttle tests.
Purpose of the Study:
- To examine changes in performance capacity during repeated 3-minute all-out shuttle tests.
- To analyze physiological responses, including heart rate and blood lactate, to these repeated efforts.
Main Methods:
- Ten participants completed maximal treadmill tests for aerobic capacity assessment.
- Participants performed three 3-minute all-out shuttle tests to calculate critical velocity (CV), anaerobic work capacity (D'), and maximal sprint speed (S MAX).
- Heart rate and blood lactate levels were measured to assess physiological strain.
Main Results:
- Critical velocity (CV) did not significantly change across repeated bouts.
- Anaerobic work capacity (D') and maximal sprint speed (S MAX) significantly decreased with repeated efforts.
- Maximal aerobic capacity (V̇ o2 peak) was significantly correlated with CV in both the first and third bouts.
Conclusions:
- Critical velocity may represent a repeatable performance threshold.
- The capacity to exceed critical velocity diminishes significantly with repeated all-out efforts.
- Further research is needed to identify strategies for improving the repeatability of anaerobic work capacity (D').

