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Updated: Sep 20, 2025
07:52
In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
Published on: November 2, 2020
6.6K
In Vivo CRISPR Interference Screen Reveals Long Noncoding RNA Portfolio Crucial for Cutaneous Squamous Cell Carcinoma
Gyuhyeon Kim1, Zurab Siprashvili2, Xue Yang2
1Program in Epithelial Biology, Department of Dermatology, Stanford University School of Medicine, Stanford, California, USA; Department of Biochemistry, Stanford University School of Medicine, Stanford, California, USA.
The Journal of Investigative Dermatology
|May 29, 2025
Summary
This study identifies long noncoding RNAs (lncRNAs) that regulate the growth of cutaneous squamous cell carcinoma (cSCC). LINC00704 and LINC01116 are highlighted as key players and potential biomarkers for this common skin cancer.
Area of Science:
- Oncology
- Molecular Biology
- Dermatology
Background:
- Cutaneous squamous cell carcinoma (cSCC) is a significant cause of skin cancer mortality.
- Understanding novel molecular mechanisms driving cSCC growth is crucial.
- The role of long noncoding RNAs (lncRNAs) in cSCC remains largely unexplored.
Purpose of the Study:
- To identify lncRNAs involved in regulating cSCC development.
- To characterize the function of specific lncRNAs in cSCC cell proliferation.
- To explore potential lncRNA biomarkers for cSCC.
Main Methods:
- Pseudobulk analysis of single-cell sequencing data from normal and cSCC human skin.
- CRISPR interference screens in vitro.
- Xenograft models for in vivo validation.
Main Results:
- A global portfolio of keratinocyte-specific lncRNAs in cSCC was determined.
- Several lncRNAs were identified that impact cSCC growth in vitro and in vivo.
- LINC00704 and LINC01116 were validated as proliferation-regulating lncRNAs in cSCC.
Conclusions:
- This study provides a comprehensive signature of lncRNAs implicated in cSCC growth regulation.
- LINC00704 and LINC01116 show potential as biomarkers for cSCC.
- lncRNAs represent a novel class of therapeutic targets for cSCC.
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