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Chromosome-level genome assembly of Paracoccus marginatus based on PacBio and Hi-C technologies
Jiufeng Wei1, Jinying Xue1, Xuejie Shen1
1College of Plant Protection, Shanxi Agricultural University, Jinzhong, Shanxi, 030801, China.
Scientific Data
|May 29, 2025
Summary
The papaya mealybug genome was sequenced, providing a vital resource for understanding and managing this invasive pest. This high-quality assembly aids research in population genetics and evolutionary studies.
Area of Science:
- Genomics
- Entomology
- Invasive Species Biology
Background:
- Invasive species, such as the papaya mealybug (Paracoccus marginatus), present significant ecological and economic challenges globally.
- Paracoccus marginatus is a widespread invasive pest affecting over 200 plant species across more than 60 countries.
Purpose of the Study:
- To assemble the chromosome-level genome of the invasive papaya mealybug (Paracoccus marginatus).
- To provide a high-quality genomic resource for research into invasive species, population genetics, and insect evolution.
Main Methods:
- Genome sequencing utilizing PacBio and Hi-C technologies.
- Bioinformatic analysis for genome assembly, annotation, and quality assessment (BUSCO, CEGMA).
Main Results:
- A chromosome-level genome assembly of 213.81 Mb, organized into four chromosomes.
- High contig (20.2 Mb) and scaffold (48.01 Mb) N50 values, indicating a contiguous assembly.
- Genome completeness of 95.5% (BUSCO) and 99.56% annotated genes (CEGMA), with 13,367 protein-coding genes identified.
- Repetitive sequences constitute 49.26% of the assembled genome.
Conclusions:
- The high-quality P. marginatus genome assembly is a crucial resource for advancing research in invasive species management.
- This genomic data will facilitate comparative genomics within Hemiptera and support evolutionary studies.
- The assembly provides a foundation for future investigations into the genetic basis of pest invasiveness and adaptation.
