Related Experiment Video
Updated: Jan 28, 2026
11:40
Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
Published on: June 23, 2022
3.0K
The study of plasmodesmal biology using proximity labeling technologies.
1Department of Genetics, Development, and Cell Biology, Iowa State University, Ames, IA 50011, USA.
Journal of Experimental Botany
|May 30, 2025
Summary
Plasmodesmata (PD) are crucial for plant cell communication. New proximity labeling assays help map PD proteins, advancing our understanding of their function and regulation.
Area of Science:
- Plant Biology
- Cellular Communication
- Molecular Biology
Background:
- Plasmodesmata (PD) are vital for intercellular transport in plants.
- Understanding PD proteomes is key to plant cell communication.
- Previous research identified PD-associated proteins.
Purpose of the Study:
- To review past efforts in uncovering PD proteomes.
- To highlight recent advances using proximity labeling (PL) assays.
- To emphasize the potential of PL assays in PD biology.
Main Methods:
- Review of existing literature on PD proteomes.
- Application of proximity labeling (PL) assays.
- Mapping plant protein interactomes related to PD.
Main Results:
- Significant progress in identifying PD-associated proteins.
- PL assays offer novel insights into protein interactions at PD.
- PL assays enhance understanding of PD composition and dynamics.
Conclusions:
- PL assays are powerful tools for studying PD biology.
- Integrating PL with other methods provides comprehensive insights.
- Future research can deepen understanding of PD function and regulation.
Related Concept Videos
01:57What is Conservation Biology?
24.3K
Conservation biology is a scientific field that focuses on the preservation of biodiversity in order to protect ecosystems while meeting the needs of the human population. Humans require properly functioning ecosystems to maintain our supply of natural resources, including food, medicines, and building materials.
24.3K
01:31Hybridoma Technology
17.5K
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
17.5K
02:59Biological Effects of Radiation
17.8K
All radioactive nuclides emit high-energy particles or electromagnetic waves. When this radiation encounters living cells, it can cause heating, break chemical bonds, or ionize molecules. The most serious biological damage results when these radioactive emissions fragment or ionize molecules. For example, α and β particles emitted from nuclear decay reactions possess much higher energies than ordinary chemical bond energies. When these particles strike and penetrate matter, they...
17.8K
01:22Factors Influencing Attraction I: Proximity
260
Proximity plays a fundamental role in shaping interpersonal attraction by increasing opportunities for interaction and fostering familiarity. Research consistently demonstrates that individuals are more likely to form social bonds with those who are physically closer to them, whether in residential settings, workplaces, or educational institutions. This effect is largely driven by the increased frequency of encounters, which facilitates the development of friendships and romantic...
260
02:55Synthetic Biology
5.6K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
Golden rice
Golden rice is a genetically modified...
5.6K
01:29Biological Causes of Schizophrenia
572
Schizophrenia, a severe psychiatric disorder, arises from a complex interplay of biological factors, including genetic predisposition, structural brain abnormalities, neurotransmitter dysregulation, and developmental irregularities. These factors collectively contribute to the onset and progression of the disorder, which typically manifests in late adolescence or early adulthood.
Genetic Factors in Schizophrenia
The genetic basis of schizophrenia is strongly supported by family and twin...
Genetic Factors in Schizophrenia
The genetic basis of schizophrenia is strongly supported by family and twin...
572