Related Experiment Video

Updated: Jan 18, 2026

Fabrication of Tongue Extracellular Matrix and Reconstitution of Tongue Squamous Cell Carcinoma In Vitro
08:15

Fabrication of Tongue Extracellular Matrix and Reconstitution of Tongue Squamous Cell Carcinoma In Vitro

Published on: June 20, 2018

6.6K

FOXA1 Transcriptional Repression of PLSCR1 Inhibits Tongue Squamous Cell Carcinoma Progression

Jinxin Wang1, Cong Zhang1, Junrong Wang1

  • 1Department of Head and Neck Surgery, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, P.R. China.

Cell Biochemistry and Biophysics
|May 30, 2025
PubMed

Insights

Forkhead box protein A1 (FOXA1) suppresses tongue squamous cell carcinoma (TSCC) by inhibiting phospholipid scramblase 1 (PLSCR1) expression. This discovery offers new therapeutic targets for head and neck cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Tongue squamous cell carcinoma (TSCC) presents limited therapeutic options.
  • Phospholipid scramblase 1 (PLSCR1) and Forkhead box protein A1 (FOXA1) roles in TSCC are not fully understood.

Purpose of the Study:

  • To investigate the expression patterns and functional relationship between PLSCR1 and FOXA1 in TSCC progression.
  • To elucidate the regulatory mechanism of PLSCR1 by FOXA1 in TSCC.

Main Methods:

  • GEO dataset analysis for PLSCR1 screening.
  • Bioinformatics analysis for upstream mechanisms.
  • Chromatin immunoprecipitation and dual-luciferase assays to detect FOXA1-PLSCR1 promoter interaction.
  • In vitro cell assays and in vivo xenograft models to assess TSCC malignant phenotypes.

Main Results:

  • PLSCR1 was significantly upregulated in TSCC, while FOXA1 was downregulated.
  • PLSCR1 knockdown suppressed TSCC cell proliferation, migration, and invasion, and increased apoptosis.
  • FOXA1 overexpression repressed TSCC malignant behaviors.
  • FOXA1 directly binds to the PLSCR1 promoter and transcriptionally inhibits its expression.
  • FOXA1's tumor-suppressive role was confirmed in rescue experiments.

Conclusions:

  • FOXA1 acts as a tumor suppressor in TSCC by transcriptionally inhibiting PLSCR1.
  • Targeting the FOXA1-PLSCR1 axis may offer a novel therapeutic strategy for TSCC.
Keywords:
FOXA1PLSCR1Tongue squamous cell carcinomaTranscription

More Related Videos

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.7K
In Vitro Establishment of a Genetically Engineered Murine Head and Neck Cancer Cell Line using an Adeno-Associated Virus-Cas9 System05:45

In Vitro Establishment of a Genetically Engineered Murine Head and Neck Cancer Cell Line using an Adeno-Associated Virus-Cas9 System

Published on: January 9, 2020

8.4K

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.1K
Eukaryotic Transcription Inhibitors01:52

Eukaryotic Transcription Inhibitors

Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
10.9K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
8.4K
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
5.9K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
7.3K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

3.9K