2D and 3D In Vitro Co-culture for Cancer and Bone Cell Interaction Studies
Ryan T Bishop1, Jesus Delgado-Calle2,3, Michaela R Reagan4
1Department of Tumor Microenvironment and Metastasis, H. Lee Moffitt Cancer Research Center and Institute, Tampa, FL, USA.
Methods in Molecular Biology (Clifton, N.J.)
|May 31, 2025
Abstract:
Co-culture assays are used to study the mutual interaction between cells in vitro. This chapter describes 2D and 3D co-culture systems used to study cell-cell signaling crosstalk between cancer cells and bone marrow adipocytes, osteoblasts, osteoclasts, and osteocytes. The chapter provides a step-by-step guide to the most used cell culture techniques, functional assays, and gene expression.
