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Updated: Aug 7, 2026
Assessing Changes in Volatile General Anesthetic Sensitivity of Mice after Local or Systemic Pharmacological Intervention
Published on: October 16, 2013
Overexpression of colonic VIP ameliorates cognitive function and barrier system damage caused by sevoflurane
Huihui Liao1, Xinyi Wang2, Chenyi Yang3
1The Third Central Clinical College of Tianjin Medical University, Tianjin 300170, China; Department of Anesthesiology, The Third Central Hospital of Tianjin, 83 Jintang Road, Hedong District, Tianjin 300170, China; The Third Central Hospital of Tianjin, 83 Jintang Road, Hedong District, Tianjin 300170, China; Tianjin Key Laboratory of Extracorporeal Life Support for Critical Diseases, Tianjin, China; Artificial Cell Engineering Technology Research Center, Tianjin, China.
Background:
Surgery and anesthesia may compromise fragile brain function in the elderly, potentially precipitating cognitive decline or Alzheimer's disease (AD) following perioperative neurocognitive disorder (PND). Vasoactive intestinal peptide (VIP) exhibits anti-inflammatory properties via the gut-brain axis. Nevertheless, whether VIP regulation enhances cognitive function in the fragile brains of aged rat and the underlying mechanisms remain to be elucidated.
Methods:
A model of fragile brain function was established in elderly rats through bilateral common carotid artery ligation. Cognitive impairment induced by anesthesia and surgery was evaluated using behavioral assays, quantification of cognition-related protein levels, analysis of inflammatory markers, assessment of microglial polarization, and examination of intestinal mucosal and blood-brain barrier integrity. Adeno-associated virus (AAV) was employed to upregulate VIP expression in the colon, and subsequent alterations in the aforementioned parameters were recorded.
Results:
Cognitive performance was evaluated through behavioral assays, while cognition-related protein levels were determined using Western blot analysis. Anesthesia and surgical interventions exacerbated cognitive deficits in rats with fragile brain function. VIP upregulation significantly ameliorated post-anesthesia and surgery cognitive deficits, normalized cognition-related protein expression, attenuated the inflammatory response, and promoted the repair of both intestinal mucosal and blood-brain barriers.
Conclusion:
These findings indicate that VIP upregulation mitigates anesthesia- and surgery-induced cognitive impairment and its progression in aged rats with fragile brain function a, primarily by modulating the inflammatory response and preserving the gut and blood-brain barrier integrity. This study offers valuable insights potential preventive strategies against cognitive decline.
