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Transcriptomics data for muscle development in Goats.

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|June 2, 2025
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This summary is machine-generated.

This study mapped the goat transcriptome in key muscles, identifying gene expression and regulatory elements. These findings offer insights into muscle development and improving meat quality through precision breeding.

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Area of Science:

  • Livestock Genomics
  • Transcriptomics
  • Animal Science

Background:

  • Goat meat is a vital global food source, with muscle quality impacting nutrition and digestibility.
  • The longissimus dorsi and biceps femoris muscles are key determinants of meat quality and goat locomotion.

Purpose of the Study:

  • To investigate gene expression patterns and regulatory mechanisms in goat muscle development.
  • To generate a comprehensive full-length goat transcriptome dataset.

Main Methods:

  • Collected longissimus dorsi and biceps femoris muscles from pregnant goats and fetuses.
  • Performed full-length transcriptome sequencing using Oxford Nanopore Technologies (ONT).
  • Analyzed sequence data to identify transcripts, alternative splicing events, and long non-coding RNAs (lncRNAs).

Main Results:

  • Generated over 169 million valid reads, aligning 127 million to the reference genome.
  • Identified 58,092 full-length transcripts, with 50,338 annotated.
  • Discovered 89,468 alternative splicing events and predicted 5,538 lncRNAs.

Conclusions:

  • This study significantly expands the goat transcriptome database.
  • Provides critical data for understanding goat muscle development, epigenetic regulation, and meat quality.
  • Facilitates precision breeding strategies for sustainable meat goat production.