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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

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Related Experiment Video

Updated: Jun 3, 2026

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
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CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

Published on: May 30, 2025

Programmable RNA acetylation with CRISPR-Cas13.

Jihwan Yu1, Juae Jin1, Eury Kwon1

  • 1Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea.

Nature Chemical Biology
|June 2, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed a new tool to precisely add N4-acetylcytidine (ac4C) modifications to specific RNA molecules. This breakthrough enables the study of ac4C's role in cell physiology and disease.

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Last Updated: Jun 3, 2026

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
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Published on: May 30, 2025

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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

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Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

Area of Science:

  • Molecular Biology
  • RNA Biology
  • Biochemistry

Background:

  • N4-acetylcytidine (ac4C) RNA modification is linked to vital regulatory functions, including translation efficiency and RNA stability.
  • Current limitations in selectively acetylating RNA hinder understanding of ac4C's physiological relevance.

Purpose of the Study:

  • To engineer a molecular tool for targeted RNA acetylation.
  • To investigate the functional consequences of specific RNA acetylation in vivo.

Main Methods:

  • Protein engineering of N-acetyltransferase 10 (NAT10) to create a hyperactive variant (eNAT10).
  • Fusion of eNAT10 with the dCas13 RNA-targeting system for programmable RNA acetylation.
  • Utilizing dual-adeno-associated virus for in vivo delivery of programmable RNA chemical modification.

Main Results:

  • Demonstrated robust and specific acetylation of various target RNAs using the dCas13-eNAT10 system, dependent on guide RNA.
  • Showcased the ability to perform programmable RNA chemical modification in vivo.
  • Observed that RNA acetylation may influence the subcellular localization of modified transcripts.

Conclusions:

  • The developed dCas13-eNAT10 system provides an efficient tool for targeted RNA ac4C modification.
  • This tool facilitates the study of ac4C functions in diverse cellular and disease contexts.
  • The findings suggest a role for RNA acetylation in regulating transcript localization.