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Updated: Sep 19, 2025

Whole-Cell Recording of Calcium Release-Activated Calcium CRAC Currents in Human T Lymphocytes
Published on: December 21, 2010
CRAC channels and patho-physiology of peripheral organ systems
Rajesh Bhardwaj1, Anant B Parekh1
1Molecular & Cellular Biology Laboratory, National Institute of Environmental Health Sciences, National Institute of Health, Durham, USA.
None:
A rise in cytosolic Ca2+ is used as a key signalling messenger in eukaryotic cells. The Ca2+ signal drives life and death and controls myriad responses in between. Inherent in the use of such a multifarious signal is the danger of disease, arising from dysregulated Ca2+ signalling. One ancient, highly conserved and widespread Ca2+ entry pathway is the store-operated Ca2+ release-activated Ca2+ (CRAC) channel. Mutations in STIM1 and ORAI1, the genes that encode the functional channel, are tightly linked to a CRAC channelopathy in humans, which encompasses severe combined immune deficiency, myopathy and anhidrotic ectodermal dysplasia. Moreover, sustained Ca2+ entry through the channels leads to a range of systemic disorders, including acute pancreatitis, asthma and inflammatory bowel disease. In this review, we describe how aberrant CRAC channel activity causes a range of diseases, highlighting commonalities between these diverse pathologies.
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