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Updated: Sep 19, 2025
Isolation of Extracellular Vesicles from Murine Bronchoalveolar Lavage Fluid Using an Ultrafiltration Centrifugation Technique
Published on: November 9, 2018
Protocol for isolating extracellular vesicles from human or mouse airway macrophages for functional assays and in
Ezgi Sari1, Robert P Richter2, Delores A Stacks3
1University of Alabama at Birmingham, Graduate Biomedical Sciences, Birmingham, AL 35233, USA; Department of Pathology, University of Alabama at Birmingham Heersink School of Medicine, Birmingham, AL 35233, USA.
Abstract:
Macrophages are key players in host defense and lung injury, and their crosstalk with other cells dictates the course of the inflammatory response and tissue remodeling. Here, we present a protocol for isolating extracellular vesicles (EVs) from human bronchoalveolar lavage fluid (BALF) and for generating EVs from primary human or murine alveolar macrophages following stimulation by cigarette smoke extract. Furthermore, we describe how to measure matrix metalloproteinase (MMP)-12 activity on macrophage-derived EVs using a Förster resonance energy transfer assay.
