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Constraints on flagellar rotation.

S Khan, M Meister, H C Berg

    Journal of Molecular Biology
    |August 20, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Streptococcus cell rotation is hindered by energy barriers at low protonmotive force (delta p). Above a threshold, these barriers disappear, allowing free rotation and Brownian motion.

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    Area of Science:

    • Microbiology
    • Cellular Biophysics

    Background:

    • Protonmotive force (delta p) drives bacterial motility.
    • Understanding energy barriers in cell rotation is crucial for cell mechanics.

    Purpose of the Study:

    • To analyze the motion of tethered Streptococcus cells at low protonmotive force (delta p).
    • To investigate the influence of energy barriers on cell rotation.

    Main Methods:

    • Tethered cell analysis of Streptococcus.
    • Controlled energization and de-energization cycles.
    • Observation of cell stopping positions at discrete angular intervals.

    Main Results:

    • Cell rotation exhibited discrete angular positions, suggesting rotational symmetry of barriers (order 5 or 6) at low delta p.

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  • Barrier constraints were observed during gradual energization from a starved state, but not during de-energization.
  • At delta p > -30 mV, cells rotated freely, exhibiting rotational Brownian movement, indicating the motor determines torque rather than fixing position.

    • Conclusions:

    • Energy barriers significantly influence Streptococcus cell rotation at low protonmotive force.
    • The behavior of the motor changes above a specific energy threshold, allowing free rotation.