Nanopore-Based Single-Molecule Logic Unit (sMOLU)

DNA computing has recently advanced from theoretical computations to a wide variety of applications. Single-molecule DNA computing combined with nanopore technology offers a unique approach to real-time molecular computation. A single-molecule logic unit (sMOLU) is developed that uses α-hemolysin nanopores to implement a DNA-based AND logic gate within lipid bilayers. This molecular system leverages a three-way DNA junction immobilized within a nanopore on a lipid membrane, enabling enzyme-specific cleavage and signal amplification in giant unilamellar vesicles (GUVs). Fluorescence and electrophysiological measurements confirm precise logic gate operations at the single-molecule level. The sMOLU platform demonstrates the potential for advancing nanoscale computational systems with precise molecular analysis capability by integrating DNA computing and nanopore technologies.