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Light-Addressable Regenerative Photoelectrochemical Biosensor Array with Self-Calibration for High-Throughput and
Zhaopeng Liu1, Jian Li1, Liming Gao2
1State Key Laboratory of Metastable Materials Science and Technology, Nano-Biotechnology Key Lab of Hebei Province, College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China.
Analytical Chemistry
|June 5, 2025
Summary
This study introduces a self-calibrating light-addressable photoelectrochemical biosensor array (LAPECBA) for accurate detection of circulating tumor cells (CTCs). The novel biosensor minimizes interference, enabling high-throughput cancer diagnostics.
Area of Science:
- Biomaterials Science
- Analytical Chemistry
- Cancer Diagnostics
Background:
- Circulating tumor cells (CTCs) are crucial biomarkers for real-time cancer monitoring.
- Light-addressable photoelectrochemical biosensor arrays (LAPECBA) offer rapid, high-throughput CTC detection.
- Existing LAPECBA methods face challenges with background interference, baseline drift, and batch variability.
Purpose of the Study:
- To develop a self-calibrating LAPECBA for enhanced accuracy and high-throughput detection of CTCs.
- To overcome limitations of conventional LAPECBA, including interference and drift.
- To enable sensitive and reliable CTC quantification for clinical applications.
Main Methods:
- Fabrication of an α-Fe2O3/Bi2S3 heterojunction electrode for improved photoelectrochemical performance.
- Construction of an aptamer-DNA concatemer (ADC) interface for efficient CTC capture and ATP-triggered release.
- Implementation of a self-calibration strategy using photocurrent differences to correct for background noise and drift.
- Light-addressable multiplexed detection utilizing a laser pen on a single FTO electrode.
Main Results:
- The developed LAPECBA achieved a linear detection range of 50-1000 cells/mL for CTCs.
- A low limit of detection of 2 cells/mL (S/N = 3) was demonstrated.
- The self-calibration strategy effectively reduced background interference, baseline drift, and batch deviation.
- ATP-stimulated release of captured CTCs was achieved, showcasing the system's regenerative capability.
Conclusions:
- The self-calibrating LAPECBA offers a robust platform for high-throughput and accurate CTC detection.
- This technology has significant potential to improve real-time cancer diagnosis and therapeutic monitoring.
- The ability to release CTCs facilitates downstream molecular analysis, enhancing clinical utility.
