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Fe-Doped Carbon Dots Functionalized Nanoelectrode for In Situ Monitoring of Metabolism-Associated ROS Changes in
Hong Jiang1, Ya-Qin Wang1, Xiao-Yang Dong1
1State Key Laboratory of Metastable Materials Science and Technology, Nano-Biotechnology Key Lab of Hebei Province, School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China.
Analytical Chemistry
|March 18, 2026
Summary
Researchers developed a nanoelectrode to monitor reactive oxygen species (ROS) in single microglia during Alzheimer
Area of Science:
- Biomedical Engineering
- Neuroscience
- Metabolic Research
Background:
- Reactive oxygen species (ROS) are crucial signaling molecules in metabolic reprogramming, necessitating in situ monitoring during cellular changes.
- Neuroinflammation in Alzheimer's disease involves altered metabolic states and ROS dynamics in microglia.
Purpose of the Study:
- To develop a method for real-time, single-cell monitoring of intracellular ROS during metabolic reprogramming in microglia.
- To investigate the role of cordycepin in modulating microglial metabolism and ROS levels under neuroinflammatory conditions.
Main Methods:
- Fabrication of an Fe-doped carbon dots (FeCDs)-functionalized nanoelectrode for sensitive ROS detection.
- In situ monitoring of intracellular ROS in single microglia within a neuroinflammatory environment mimicking Alzheimer's disease.
- Assessment of cordycepin's effect on microglial metabolism and ROS production.
Main Results:
- The FeCDs-functionalized nanoelectrode successfully achieved in situ monitoring of intracellular ROS in single microglia.
- Cordycepin was found to target hexokinase II, inducing metabolic reprogramming in microglia.
- Cordycepin treatment mitigated the elevation of intracellular ROS induced by LPS + Aβ stimulation.
Conclusions:
- This study presents an effective strategy for single-cell level in situ ROS monitoring during metabolic reprogramming.
- The findings highlight cordycepin's potential as a therapeutic agent by modulating microglial metabolism and reducing oxidative stress in Alzheimer's disease models.
- This approach is vital for advancing disease diagnosis and therapeutic development in neuroinflammatory conditions.
