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Updated: Mar 19, 2026
Quantification of Reactive Oxygen Species Using 2′,7′-Dichlorofluorescein Diacetate Probe and Flow-Cytometry in Müller Glial Cells
Published on: May 13, 2022
Fe-Doped Carbon Dots Functionalized Nanoelectrode for In Situ Monitoring of Metabolism-Associated ROS Changes in
Hong Jiang1, Ya-Qin Wang1, Xiao-Yang Dong1
1State Key Laboratory of Metastable Materials Science and Technology, Nano-Biotechnology Key Lab of Hebei Province, School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China.
Abstract:
In metabolic reprogramming, reactive oxygen species (ROS) serve not only as harmful byproducts but also as essential signaling molecules that drive reprogramming processes to establish new metabolic homeostasis. Therefore, the in situ monitoring of ROS changes during metabolic reprogramming is crucial for understanding metabolic changes. Herein, we develop an Fe-doped carbon dots (FeCDs)-functionalized nanoelectrode that exploits the excellent catalytic activity of FeCDs to achieve in situ monitoring of intracellular ROS in single microglia under a neuroinflammatory microenvironment mimicking Alzheimer's disease. Further investigations reveal that cordycepin may target hexokinase II, a key regulatory enzyme in glucose metabolism, thereby inducing metabolic reprogramming in microglia and mitigating the LPS + Aβ-induced elevation in intracellular ROS. This work provides an efficient strategy for in situ monitoring of ROS changes during metabolic reprogramming at the single-cell level, which is vital for advancing disease diagnosis and therapeutic development.
