An Entropy-Driven Multipedal DNA Walker Microsensor for In Situ Electrochemical Detection of ATP

Area of Science:

• Biomedical Engineering
• Electrochemistry
• Molecular Biology

Background:

• Micro- and nanoelectrode electrochemistry are vital for in vivo biomolecule monitoring.
• Challenges include sensitive detection of nonelectroactive molecules and specific detection in complex biological settings.

Purpose of the Study:

• To develop a highly sensitive and selective electrochemical microsensor for adenosine triphosphate (ATP) detection.
• To utilize an entropy-driven multipedal DNA walker for enhanced signal amplification.

Main Methods:

• Immobilized ATP aptamer on a microelectrode surface to create a DNA walker track.
• Developed a multipedal DNA walker with quantum dots for autonomous movement.
• Utilized methylene blue (MB) for signal amplification upon ATP binding and walker movement.

Main Results:

• The microsensor achieved highly sensitive and selective ATP detection.
• Demonstrated successful in situ monitoring of ATP release from BV2 cells under hypotonic conditions.
• Confirmed that increased cell volume enhances ATP release.

Conclusions:

• The multipedal DNA walker microsensor provides a novel strategy for in vivo detection of nonelectroactive biomolecules.
• This technology is crucial for investigating neurochemical processes in neurological diseases.
• The sensor offers a sensitive and selective approach for real-time biomolecule monitoring.