A pooled testing strategy for enterovirus D68 to facilitate local, resource-conserving surveillance
Madeline E Spradley1, Timothy Williams2, Rendie McHenry3
1Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA.
Diagnostic Microbiology and Infectious Disease
|June 5, 2025
Abstract
None:
Pooled testing represents a resource-conserving diagnostic strategy, and we propose enterovirus-D68 (EV-D68) as an attractive target for pooling (especially locally). To validate an RT-qPCR-based approach, retrospective pools were contrived from EV-D68-positive nasopharyngeal specimens and non-EV-D68-enterovirus-positive specimens. Five/nine-fold pools demonstrated 90.9 %/88.6 % sensitivity versus-unpooled testing (no false-positives), with commensurate results observed prospectively.
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