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Updated: Jun 13, 2026

Measuring Left Ventricular Pressure in Late Embryonic and Neonatal Mice
Published on: February 23, 2012
Embryonic cerebrospinal fluid pressure in fetal mice in utero: External factors pressurize the intraventricular space
Koichiro Tsujikawa1, Reina Muramatsu1, Takaki Miyata1
1Department of Anatomy and Cell Biology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Background:
Previous experiments inducing leakage of embryonic cerebrospinal fluid (CSF) suggest the necessity of intraventricular CSF pressure (PCSF) for brain morphogenesis. Nevertheless, how embryonic PCSF occurs is unclear, especially in utero.
Results:
Using a Landis water manometer, we measured PCSF in fetal mice isolated from the amniotic cavity (PCSF-ISO) and found that PCSF-ISO rose from 20 Pa at embryonic day (E) 10 to 100-110 Pa at E14-16. At E13, intraventricular injections of ≥3 μL of saline elevated PCSF-ISO by ~30%, whereas those of inhibitors of CSF secretion decreased PCSF-ISO by ~30%. Shh-mediated cerebral wall expansion did not significantly increase PCSF-ISO. Removal of the brain-surrounding contractile tissues decreased PCSF-ISO by 80%-90%. We then found that the intraamniotic pressure measured in utero (PAF-IU) declined from 2000 Pa at E10 to 500 Pa at E15-18 but was always much greater than PCSF-ISO. Direct measurement of PCSF in utero (PCSF-IU) at E13 and E15 coupled with the measurement of PCSF-ISO under hydrostatic pressure loading to mimic PAF-IU at various embryonic ages revealed the following relationship: PCSF-IU = PCSF-ISO + PAF-IU.
Conclusions:
The PCSF of mice in utero is influenced by external factors, most strongly by intraamniotic pressure and less strongly by brain-confining tissues.
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