Hematological response of immunized and serially bled Japanese quail

Sexually mature female Japanese quail (Coturnix coturnix japonica) were immunized by an intravenous injection with .5 ml of a 7.5% suspension of Chukar partridge (Alectoris graeca chukar) red blood cells (CRBC). Blood samples were collected either serially from one group of quail or a single time from separate groups of quail at 0 (nonimmunized), 3, 6, and 9 days post-immunization. Total anti-CRBC hemagglutinin titers (HA) were measured by a microhemagglutinin procedure. Selected hematological variables were also measured. Mean HA was not affected by serial blood sampling. Total erythrocyte numbers, percent hematocrit, and hemoglobin levels were depressed in serially bled quail at 3 days postimmunization. Reticulocytosis was found in serially bled quail at 6 days postimmunization.