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Updated: Sep 19, 2025

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Complete genome sequence of a novel narnavirus with inverted complementary termini from the rice blast fungus
Cong Li1,2, Yuxin Wu2, Xinyi Li2
1State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Abstract:
A novel single-stranded (+ss) RNA mycovirus, designated as "Magnaporthe oryzae narnavirus 2" (MoNV2), was identified in the rice blast fungus Magnaporthe oryzae isolate NJ471. MoNV2 has an RNA genome of 3,086 nucleotides, which contains a single open reading frame (ORF) that is predicted to encode an RNA-dependent RNA polymerase (RdRp). Genome sequence comparisons and phylogenetic analysis suggested that MoNV2 is a new member of the genus Narnavirus of the family Narnaviridae. The 5' and 3' ends of the MoNV2 genomic RNA are complementary to each other, potentially forming a panhandle structure.
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