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Updated: Jan 14, 2026

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Full-length genome sequence of Magnaporthe oryzae botourmiavirus 13
Yuxin Wu1, Miaomiao Chen1, Cong Li1
1College of Agriculture, Yangtze University, Jingzhou, Hubei, 434025, China.
Abstract:
Through virome sequence analysis of the rice blast fungus Magnaporthe oryzae strain HM278, a novel positive-sense single-stranded RNA (+ssRNA) virus was identified and designated as "Magnaporthe oryzae botourmiavirus 13" (MoBV13). The complete genome of MoBV13 is 2,143 nucleotides in length and contains a single open reading frame (ORF) encoding a putative RNA-dependent RNA polymerase (RdRp), consisting of 568 amino acids, with a molecular mass of 62.5 kDa. Sequence comparisons and phylogenetic analysis indicated that MoBV13 is a novel member of the genus Botoulivirus of the family Botourmiaviridae.
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