Related Experiment Video

Updated: Sep 19, 2025

Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae
09:25

Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae

Published on: June 2, 2021

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FK506 Targets MoFpr1 to Modulate Autophagy and Ubiquitination, Inhibiting the Pathogenicity of Magnaporthe oryzae

Wen-Hui Zhao1, Jiong-Yi Yan1,2, Ke-Xin Xie1

  • 1State Key Laboratory for Quality and Safety of Agro - Products, Zhejiang Provincial Key Laboratory of Agricultural Microbiomics, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.

Journal of Agricultural and Food Chemistry
|June 10, 2025
PubMed

Insights

The immunosuppressant FK506 effectively controls rice blast disease by inhibiting Magnaporthe oryzae growth and pathogenicity. It targets MoFpr1, impacting autophagy and ubiquitination for sustainable crop protection.

Area of Science:

  • Plant Pathology
  • Mycology
  • Molecular Biology

Background:

  • Rice blast, caused by *Magnaporthe oryzae*, poses a significant threat to global rice production.
  • Developing novel antifungal agents is crucial for sustainable agriculture and food security.

Purpose of the Study:

  • To investigate the antifungal potential of FK506 against *Magnaporthe oryzae*.
  • To identify the molecular target of FK506 in *M. oryzae* and elucidate its mechanism of action.

Main Methods:

  • Mycelial growth inhibition assays
  • Pathogenicity assays
  • Transcriptomic and metabolomic analyses
  • X-ray crystallography and site-directed mutagenesis
  • Broad-spectrum antifungal screening
  • Field trials

Main Results:

  • FK506 significantly inhibited mycelial growth, appressorium formation, and pathogenicity of *M. oryzae*.
  • MoFpr1 (FK506-binding protein 1B) was identified as the direct target of FK506.
  • FK506 disrupts autophagy and ubiquitination pathways in *M. oryzae*.
  • Structural studies confirmed the FK506-MoFpr1 interaction.
  • FK506 showed broad-spectrum antifungal activity and effective rice blast control with low phytotoxicity.

Conclusions:

  • FK506 is a potent antifungal agent against *Magnaporthe oryzae*.
  • The MoFpr1-dependent inhibition of autophagy and ubiquitination is key to FK506's antifungal action.
  • FK506 holds promise for sustainable management of rice blast and other plant diseases.
Keywords:
FK506X-ray crystallographybroad-spectrum antifungal activityrice blast

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