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Quantitative analysis of the composition of mixtures of one-chain and two-chain tissue-type plasminogen activator
Thrombosis Research
|August 1, 1985
Abstract:
Tissue-type plasminogen activator (t-PA) exists in two active forms, one-chain t-PA and two-chain t-PA. These two forms differ in their kinetic constants for certain substrates. An assay has been devised to determine the composition of unknown mixtures of both t-PA forms, using the activity measurement on two different substrates. The best results are obtained using plasminogen and less than Glu-Pro-Arg-pNA as substrates.