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Updated: Jun 12, 2025
Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization
Published on: October 6, 2019
Functional comparison of tilapia interferon regulatory factor (IRF) 1 and IRF11 in type I interferon-mediated
Meiling Zhang1, Qingqing Wen1, Shaoshuai Tang1
1Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy culture, and Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institute, College of Fishery, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, and Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings, Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China.
Abstract:
Interferon regulatory factors (IRFs) are a family of immune regulators in the production of interferons (IFNs), and IRF11 is considered as a fish-specific IRF member sharing similarities with IRF1. However, the information about the distinct functions of fish IRF1 and IRF11 is rather limited. Here, we cloned IRF1 and IRF11 genes (named as On-IRF1 and On-IRF11) from tilapia. Syntenic analysis suggested that IRF1 and IRF11 genes are two distinct IRF members located in different genomic regions. Expression analyses showed that a much higher upregulation of On-IRF1 was observed than that of On-IRF11 after poly(I:C) treatment. In addition, On-IRF1 (not On-IRF11) is a typical interferon-stimulated genes (ISGs) as seen in mammals, and On-IRF1 induction by IFNs depends on the JAK-STAT signaling. Importantly, the transfection of On-IRF1 elicited a dramatic and sustained induction of IFN and ISGs, whereas On-IRF11 triggered a transient induction with a relative low level in fish cells. Consistently, the antiviral effect of On-IRF1 was much higher than that of On-IRF11, and DBD and IAD are essential for the antiviral functions of On-IRF1 and On-IRF11. The present results thus contribute to better understanding of the functional divergence of fish IRF1 and IRF11 in type I IFNs responses.
