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Related Experiment Video

Updated: Jun 12, 2025

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
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Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

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LM-GlycoRepo Version 1.0: A Novel Repository System for Mouse Tissue Glycome Mapping Data.

Chiaki Nagai-Okatani1, Noriaki Fujita1, Patcharaporn Boottanun1,2

  • 1Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.

Journal of Proteome Research
|June 11, 2025
PubMed
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A new data repository, LM-GlycoRepo, has been developed for lectin microarray (LMA) data. This system ensures FAIR data principles for protein glycosylation research and supports data sharing during peer review.

Area of Science:

  • Glycomics
  • Biotechnology
  • Data Science

Background:

  • Lectin microarray (LMA) is a sensitive method for profiling protein glycosylation.
  • The increasing use of LMA necessitates a data repository adhering to FAIR principles (Findable, Accessible, Interoperable, Reusable).

Purpose of the Study:

  • To introduce LM-GlycoRepo, a novel repository system for lectin-based multimodal (LM) data.
  • To establish a system compliant with the MIRAGE (Minimum Information Required for a Glycomics Experiment) guideline.

Main Methods:

  • LM-GlycoRepo Version 1.0 is designed for mouse tissue glycome mapping data using laser microdissection (LMD)-assisted LMA.
  • The system accepts LMD images, LMA data, and lectin staining images.
  • An embargo system is incorporated for data release timing, compatible with manuscript peer review.
Keywords:
endogenous glycoproteinformalin-fixed paraffin-embedded tissue sectionglycoinformaticshistochemistrylaser microdissectionlectin microarrayrepository systemtissue glycome mapping

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Main Results:

  • LM-GlycoRepo Version 1.0 is specialized for mouse tissue glycome mapping data.
  • Deposited data can be visualized using the LM-GlycomeAtlas web tool after the embargo period.
  • The repository is freely accessible via the GlyCosmos portal.

Conclusions:

  • LM-GlycoRepo provides a FAIR-compliant repository for LMA and related data.
  • The system supports data sharing and visualization for glycomics research.
  • Future versions will accommodate diverse biospecimens, including human samples.