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Updated: Jun 14, 2025

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Multiparametric super-resolution optoacoustic imaging with ICG-tagged microbubbles.

Daniil Nozdriukhin1,2, Eva Remlova1,2,3, Lin Tang1,2

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Summary

We developed novel indocyanine green (ICG)-coated polyvinyl alcohol (PVA) microbubbles for enhanced localization optoacoustic tomography (LOT). This advancement enables safer, super-resolution microangiography for diagnosing circulatory conditions.

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Area of Science:

  • Biomedical Imaging
  • Nanotechnology
  • Medical Diagnostics

Background:

  • Localization optoacoustic tomography (LOT) offers super-resolution functional microangiography beyond optical microscopy limits.
  • LOT can simultaneously assess microvascular morphology, oxygen saturation, and blood flow velocity.
  • Clinical adoption of LOT is hindered by the absence of human-verified intravenous contrast agents.

Purpose of the Study:

  • To develop and validate a novel contrast agent for LOT.
  • To enable safe intravenous administration for enhanced microangiography.
  • To facilitate the clinical translation of LOT.

Main Methods:

  • Formulation of polyvinyl alcohol (PVA) microbubbles coated with indocyanine green (ICG) via layer-by-layer assembly.
  • Characterization of ICG-coated microbubbles for per-particle detection in blood.
  • In vivo LOT imaging of murine brains to assess efficacy and safety.

Main Results:

  • ICG-coated PVA microbubbles were successfully synthesized for LOT.
  • Per-particle detection was achieved despite strong background light absorption in blood.
  • LOT imaging of the murine brain demonstrated no acute toxicity.

Conclusions:

  • ICG-coated microbubbles represent a promising, safe contrast agent for LOT.
  • The established safety profiles of microbubbles and ICG support clinical translation.
  • This innovation is expected to significantly advance LOT for diagnosing microcirculatory alterations.