[Immobilized thrombolytic enzymes and their use in ophthalmology]

Soluble highly active conjugates were obtained after complex formation or covalent binding of trypsin and urokinase with polymers of various chemical structure as well as by means of condensation of the enzyme molecules as a result of which oligo- and heterooligoproteins were produced. Pharmacological activity of the conjugates obtained was studied in the course of treatment of rabbits with experimental hyphemia. All the preparations produced exhibited high therapeutic efficiency: the time of hyphemia resorption was decreased, the effect of immobilized enzymes was prolonged as compared with controls. Dependence on the molecular mass and the dose of enzymatic preparations of the period of hyphemia resorption was studied using heteromers of urokinase and hemoglobin. Maximal activity was noted with the preparations of molecular mass 4.10(5) daltons; they maintained the optimal level of pharmacological activity even after 4-fold decrease in the dose used as compared with other immobilized preparations of trypsin and urokinase. The immobilized preparations were 20-fold more effective as compared with native unmodified enzymes.