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Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
Published on: July 11, 2014
Chromatographic Choice in Cerebrospinal Fluid Metabolomics Identification Outcome : Metabolomics for Cerebrospinal
Sofía C Ayala Rodríguez1,2, Alyssa M Suarez1,3, Victor M Lu4
1Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami Integrative Metabolomics Research Center, Miami, FL, USA.
None:
Limited literature on the optimal chromatographic method for cerebrospinal fluid (CSF) extraction and identification is available. Ultraperformance liquid chromatography-mass spectrometry (UPLC-MS) is an extensively used tool in metabolic profiling because of its high resolving power, analysis sensitivity, and selectivity. This technique allows for reliable structural elucidation for metabolite identification and quantification at low micromolar to nanomolar concentration levels. In this book chapter, we discuss two complementary UPLC-MS methods, reversed-phase (RP) and chromatography hydrophilic interaction liquid chromatography (HILIC), to characterize metabolomic analysis of CSF samples.

