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Updated: Jun 14, 2025

Isolation of Murine Lymph Node Stromal Cells
05:47

Isolation of Murine Lymph Node Stromal Cells

Published on: August 19, 2014

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Identification and Mapping of Human Lymph Node Stromal Cell Subsets by Combining Single-Cell RNA Sequencing with

Cristoforo Grasso1,2,3,4, Janna E G Roet4,5, Catarina Gago de Graça4,5

  • 1Department of Rheumatology and Clinical Immunology, Amsterdam UMC, location AMC, University of Amsterdam, Amsterdam, the Netherlands.

European Journal of Immunology
|June 11, 2025
PubMed
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This summary is machine-generated.

This study reveals 10 distinct human lymph node stromal cell (LNSC) subtypes using single-cell RNA sequencing. These subtypes are spatially located in specific lymph node regions, suggesting unique roles in immune responses.

Area of Science:

  • Immunology
  • Cell Biology
  • Genomics

Background:

  • Lymph node stromal cells (LNSCs) play a key role in immune regulation.
  • The heterogeneity of human LNSCs is not fully understood.
  • Understanding LNSC diversity is crucial for comprehending immune responses within lymph nodes.

Purpose of the Study:

  • To comprehensively characterize the heterogeneity of human lymph node stromal cells.
  • To define gene signatures for distinct LNSC subtypes.
  • To investigate the spatial distribution and potential functional niches of LNSC subtypes within human lymph nodes.

Main Methods:

  • Single-cell RNA sequencing (scRNA-seq) of 9267 LNSCs from human lymph nodes.
  • Validation of identified subtypes across multiple human donors and public datasets.
Keywords:
fibroblasthumanlymph node stromal cellssingle‐cell RNA sequencingspatial transcriptomicsstroma‐immune system

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  • Integration of scRNA-seq data with spatial transcriptomic data to map LNSC locations.
  • Utilized NicheNet to explore LNSC-lymphocyte interactions.

    • Main Results:

    • Identified and characterized 10 distinct LNSC fibroblast subtypes based on gene signatures.
    • Validated the existence and gene expression profiles of these subtypes across different donors.
    • Demonstrated that each LNSC subtype is spatially restricted to specific regions within the lymph node architecture.
    • Predicted distinct LNSC-lymphocyte interactions based on subtype localization.

    Conclusions:

    • Human lymph node stromal cells exhibit significant heterogeneity, with 10 defined subtypes.
    • The spatial organization of LNSC subtypes suggests region-specific immunomodulatory functions.
    • This detailed mapping provides a foundation for future research into LNSC niches and their roles in health and disease.