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Investigating Bacterial Invasion of Highly Differentiated Human Bronchial Epithelial Cell Barriers
Hannah J Parks1, Miguel A Valvano2
1Infection Biology Group, Wellcome-Wolfson Institute for Experimental Medicine, Queen's University Belfast, Belfast, UK.
None:
Confocal microscopy provides information on host-pathogen interactions. To examine infection at the respiratory epithelial barrier, primary bronchial cells differentiated at an air-liquid interface (ALI) can be imaged and reconstructed as a 3D model. In this chapter, we describe a method for immunostaining and fluorescence imaging of well-differentiated bronchial epithelial tissue that has been exposed to the cystic fibrosis respiratory pathogen Achromobacter xylosoxidans.
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