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Updated: Jun 15, 2025
Mouse Wound Models and Preparation of Single-Cell Suspensions
Published on: September 27, 2024
Healing effects of topically applied Ocimum basilicum L. on excisional wounds in mice
Karine Sthéfany Serpa Amaral Dias1, Elisângela Elduina Ferreira1, Renan de Araújo Costa1
1Universidade Federal de São João del-Rei - Laboratory of Experimental Pathology - São João del-Rei (MG) - Brazil.
Purpose:
To evaluate the topical treatment with the ethanolic extract of Ocimum basilicum L. (OBEE) on excisional wounds in mice.
Methods:
The plant material was identified and collected, and the ethanolic extract was obtained from the aerial parts of O. basilicum. The OBEE was resuspended in saline at concentrations of 38 and 100 mg/kg for topical application on the left and right lesions, respectively. Mice were anesthetized, excisional wounds were made on the dorsal region and divided into four groups (n = 6) based on treatment duration: one, three, five, and 21 days. The control group received saline. After the treatments, the animals were euthanized, and the wounds were collected for histopathological analysis the inflammatory infiltrate, mast cell count, and deposition of newly synthesized collagen matrix.
Results:
The daily topical application of OBEE at concentrations of 38 and 100 mg/kg reduced the inflammatory response, evidenced by decreased leukocyte infiltration in the damaged tissue, and improved the deposition of newly synthesized matrix, with thicker, more intertwined collagen fibers resembling intact skin. These effects may be attributed to the phenolic compounds, tannins, flavonoids, and essential oils present in basil.
Conclusion:
OBEE shows promise as a potential healing agent in excisional wounds.
