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Updated: Jun 16, 2025

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Annexin A2 is a potential membrane protein target of cobra venom cytotoxin

Yanning Yong1, Michelle Khai Khun Yap1

  • 1School of Science, Monash University Malaysia, Bandar, Sunway, Malaysia.

Toxicon : Official Journal of the International Society on Toxinology
|June 11, 2025
PubMed

Insights

This study identifies annexin A2 as a key cell membrane target for cytotoxin (CTX), a venom component from cobras. This discovery advances understanding of how CTX causes cell damage and death.

Area of Science:

  • Biochemistry
  • Toxicology
  • Cell Biology

Background:

  • Cytotoxin (CTX) is a component of cobra venom known to induce cytotoxicity.
  • The precise mechanisms by which CTX mediates cytotoxicity remain incompletely understood.

Purpose of the Study:

  • To identify the specific cell membrane protein targets of Cytotoxin (CTX).
  • To elucidate the molecular interactions between CTX and its potential targets.

Main Methods:

  • Crosslinking differential mass spectrometry was employed to identify protein targets.
  • Binding affinity of identified complexes was quantified.

Main Results:

  • Eighty-nine proteins were identified as potential binding partners for CTX.
  • The annexin A2-CTX complex exhibited the highest stability and a high binding affinity at the nanomolar scale.

Conclusions:

  • Annexin A2 is identified as a significant cell membrane protein target for Cytotoxin (CTX).
  • The interaction between CTX and annexin A2 offers insights into CTX internalization pathways and cytotoxicity mediation.
Keywords:
Annexin A2CytotoxinDissociation constantXL-dMS

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