Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Liver Regeneration01:24

Liver Regeneration

3.2K
The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
3.2K
Hematopoiesis01:21

Hematopoiesis

5.2K
The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
5.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mesoscale maladaptation in disease organoids.

Disease models & mechanisms·2026
Same author

Designing complex organoids through developmental principles.

Cell stem cell·2026
Same author

COX2<sup>+</sup>/PDGFRα<sup>+</sup> fibroblasts selectively localize near bile ducts and interact with immune cells in early liver fibrosis in mice.

Physiological reports·2026
Same author

Large-scale and innervated functional human gut tissues for transplantation via transient spheroid confinement.

Nature biomedical engineering·2026
Same author

Vertebrate endoderm development.

Development (Cambridge, England)·2026
Same author

Peritoneal dialysis catheter complications in neonates with kidney failure.

Pediatric nephrology (Berlin, Germany)·2026
Same journal

Genetic Impacts on Variability of Body Fat Distribution Uncover Gene-Environment and Gene-Gene Interactions.

bioRxiv : the preprint server for biology·2026
Same journal

16S ribosomal RNA modification drives transcript-specific translation efficiency.

bioRxiv : the preprint server for biology·2026
Same journal

FlcE latches onto the FliL-stator complex to turbocharge flagellar motility in <i>Borrelia burgdorferi</i>.

bioRxiv : the preprint server for biology·2026
Same journal

Synaptic pruning, myelination and the emergence of psychiatric disorders in late adolescence.

bioRxiv : the preprint server for biology·2026
Same journal

Structural and functional insights into the Rcs phosphorelay.

bioRxiv : the preprint server for biology·2026
Same journal

The structural basis of RanGAP1 regulation and catalysis in nuclear transport.

bioRxiv : the preprint server for biology·2026
See all related articles

Related Experiment Video

Updated: Jun 13, 2025

Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration
08:14

Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration

Published on: May 20, 2015

8.6K

Primitive Hepatoblasts Driving Early Liver Development.

Kentaro Iwasawa1,2, Hiroyuki Koike1,2, Hasan Al Reza2,3

  • 1Division of Gastroenterology, Hepatology & Nutrition, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229-3039, USA.

Biorxiv : the Preprint Server for Biology
|June 12, 2025
PubMed
Summary
This summary is machine-generated.

Researchers discovered a new type of early liver cell, primitive hepatoblasts, originating from Cdx2-positive progenitors. These cells contribute significantly to early liver development and offer new insights into liver formation.

More Related Videos

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines
08:50

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

Published on: April 18, 2025

362
Human Liver Spheroids from Peripheral Blood for Liver Disease Studies
09:51

Human Liver Spheroids from Peripheral Blood for Liver Disease Studies

Published on: January 27, 2023

1.6K

Related Experiment Videos

Last Updated: Jun 13, 2025

Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration
08:14

Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration

Published on: May 20, 2015

8.6K
In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines
08:50

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

Published on: April 18, 2025

362
Human Liver Spheroids from Peripheral Blood for Liver Disease Studies
09:51

Human Liver Spheroids from Peripheral Blood for Liver Disease Studies

Published on: January 27, 2023

1.6K

Area of Science:

  • Developmental biology
  • Hepatogenesis
  • Cell lineage heterogeneity

Background:

  • Liver development begins with hepatoblasts from ventral foregut endoderm.
  • Understanding early liver progenitor heterogeneity is crucial for developmental insights.

Purpose of the Study:

  • To identify and characterize a novel population of early hepatoblasts.
  • To elucidate the origin and contribution of these cells during liver development.
  • To explore the regulatory mechanisms and potential functions of primitive hepatoblasts.

Main Methods:

  • Single-cell transcriptomics in mouse and human models.
  • Lineage tracing studies.
  • Epigenetic and functional perturbation assays.
  • Human pluripotent stem cell differentiation and in vivo studies.

Main Results:

  • Identification of primitive hepatoblasts from Cdx2-positive endoderm progenitors.
  • Characterization of unique molecular markers (TBX3, FGB, KRT8/18, ID3, VIM, GATA4).
  • Demonstrated significant contribution (up to 12.6%) of primitive hepatoblasts at E11.5.
  • Revealed WNT-suppression on RA-permissive CDX2+FOXA2+ progenitors primes their emergence.
  • Observed secretion of pleiotrophin and midkine by primitive hepatoblast-like cells, promoting proliferation and tissue development.

Conclusions:

  • Primitive hepatoblasts represent a distinct early hepatoblast population.
  • Their emergence is regulated by specific signaling pathways (WNT, RA).
  • These cells play a role in amplifying hepatoblast populations and forming hybrid tissues.
  • Findings provide a new framework for understanding liver lineage heterogeneity and modeling liver development.