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Related Experiment Video

Updated: Jun 10, 2026

A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells
08:30

A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells

Published on: March 4, 2020

Designing complex organoids through developmental principles.

Jonathan A Brassard1, James M Wells1

  • 1Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA; Center for Stem Cell & Organoid Medicine (CuSTOM), Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.

Cell Stem Cell
|June 8, 2026
PubMed
Summary
This summary is machine-generated.

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Next-generation organoids use developmental biology principles to create more complex models. These advanced human pluripotent stem cell (hPSC)-derived organoids better mimic human development and disease for research.

Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Organoid Technology

Background:

  • Human pluripotent stem cells (hPSCs) are differentiated into organoids, modeling human development and disease.
  • First-generation organoids lacked cellular diversity crucial for studying normal and pathological processes.

Purpose of the Study:

  • To discuss advances in creating next-generation organoids with increased cellular complexity.
  • To highlight methods for better reproducing in vivo organogenesis processes.
  • To explore engineering technologies for controlled in vitro development.

Main Methods:

  • Leveraging principles of developmental biology for directed differentiation of hPSCs.
  • Focusing on reconstructing cellular diversity within organoids.
Keywords:
assembloiddevelopmental biologyengineering organoidorganogenesisorganoidself-organization

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Vascular Organoid Generation from Human-Induced Pluripotent Stem Cells

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Last Updated: Jun 10, 2026

A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells
08:30

A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells

Published on: March 4, 2020

Vascular Organoid Generation from Human-Induced Pluripotent Stem Cells
04:41

Vascular Organoid Generation from Human-Induced Pluripotent Stem Cells

Published on: December 13, 2024

  • Investigating tissue-tissue interactions and specialized cell addition.
  • Utilizing engineering technologies to guide cell assembly.

    • Main Results:

    • Next-generation organoids exhibit enhanced cellular complexity compared to earlier models.
    • Improved recapitulation of developmental processes during organogenesis in vitro.
    • Demonstrated importance of tissue interactions and cell type incorporation.

    Conclusions:

    • Next-generation organoids offer more comprehensive models of human development and disease.
    • Advances in cellular diversity and engineering enhance organoid fidelity.
    • These improved models hold significant potential for future research.