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Updated: Aug 13, 2026

Quantification and Size-profiling of Extracellular Vesicles Using Tunable Resistive Pulse Sensing
Published on: October 19, 2014
Protocol to isolate and quantify large aging neutrophil-derived vesicles
Alan Y Hsu1, Qingxiang Huang2, Sizhou Feng2
1Department of Pathology, PhD Program in Immunology, Harvard Medical School, Mass General Brigham, Dana-Farber/Harvard Cancer Center, Boston, MA 02115, USA.
Abstract:
Large aging neutrophil-derived vesicles (LAND-Vs) are ∼1 μm ectosomes released by aging neutrophils. Here, we present a protocol to identify and purify murine LAND-Vs from in vitro neutrophil cultures and ex vivo sources, including bone marrow, blood, and bronchoalveolar lavage fluid (BALF), using centrifugation and fluorescence-activated cell sorting (FACS). We also describe steps for isolation and quantification of human LAND-Vs from isolated neutrophils and human blood. For complete details on the use and execution of this protocol, please refer to Hsu et al.1.

