Related Experiment Video
Updated: Jun 16, 2025
06:48
A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
Published on: June 16, 2022
2.0K
hnRNPH1: A Multifaceted Regulator in RNA Processing and Disease Pathogenesis.
Lijing Zhu1, Wei Yi1, Like Zhang1
1Department of Burn Surgery, Changhai Hospital, Navy Medical University, Shanghai 200433, China.
International Journal of Molecular Sciences
|June 13, 2025
Summary
Heterogeneous nuclear ribonucleoprotein H1 (hnRNPH1) is a key RNA-binding protein involved in regulating gene expression. Its dysfunction is linked to various diseases, making hnRNPH1 a potential therapeutic target.
Area of Science:
- Molecular Biology
- Biochemistry
- Genetics
Background:
- Heterogeneous nuclear ribonucleoprotein H1 (hnRNPH1) is a vital RNA-binding protein (RBP).
- It regulates RNA metabolism, including splicing, stability, and translation.
- hnRNPH1 dysfunction is implicated in numerous human diseases.
Purpose of the Study:
- To review the structural basis and molecular functions of hnRNPH1.
- To detail its mechanisms of action and pathological roles.
- To summarize therapeutic strategies and future research directions for hnRNPH1.
Main Methods:
- Literature review of existing research on hnRNPH1.
- Analysis of hnRNPH1's role in various disease models.
- Synthesis of current therapeutic approaches and challenges.
Main Results:
- hnRNPH1 binds guanine-rich RNA and G-quadruplexes.
- It influences alternative splicing, mRNA stability, and translation.
- hnRNPH1 is implicated in cancer, neurological disorders, and other pathologies.
Conclusions:
- hnRNPH1 is a significant factor in disease pathogenesis.
- It represents a promising biomarker and therapeutic target.
- Further research is needed to optimize hnRNPH1-targeted interventions.
Related Concept Videos
02:41Chromatin Structure Regulates pre-mRNA Processing
7.0K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
7.0K
02:39lncRNA - Long Non-coding RNAs
8.5K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.5K
01:23Regulation of Expression at Multiple Steps
876
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
876
02:24Regulation of Expression Occurs at Multiple Steps
22.6K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
22.6K
01:23Types of RNA
63.4K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
63.4K
02:27Nonsense-mediated mRNA Decay
10.6K
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
10.6K