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Updated: Jun 16, 2025
The In ovo CAM-assay as a Xenograft Model for Sarcoma
Published on: July 17, 2013
Implementation of a CAM Assay Using Fibrosarcoma Spheroids
Flemming Puscz1, Noah Jozsef Hatem1, Sonja Verena Schmidt1
1Department of Plastic Surgery, BG University Hospital Bergmannsheil, Ruhr University Bochum, 44789 Bochum, Germany.
This study demonstrates that fibrosarcoma spheroids can successfully grow on the chorioallantoic membrane (CAM) assay. The CAM assay provides a viable in ovo model for investigating fibrosarcoma tumor development and pathophysiology.
Area of Science:
- Oncology
- Developmental Biology
- Tissue Engineering
Background:
- Fibrosarcomas are rare, aggressive soft tissue tumors with unclear development and pathophysiology.
- The chorioallantoic membrane (CAM) assay offers an accessible in ovo model for tumor research.
Purpose of the Study:
- To determine if fibrosarcoma spheroids can grow on the CAM.
- To evaluate the CAM assay's suitability for downstream fibrosarcoma investigations.
Main Methods:
- Fertilized chicken eggs were used, and HT1080 fibrosarcoma cell spheroids (50,000, 75,000, and 100,000 cells) were applied to the CAM.
- Tumor growth, size, weight, and vascularization were assessed after 7 days.
Main Results:
- Tumor growth was confirmed in 80 out of 163 eggs (49%).
- The 100,000-cell spheroid group showed the highest tumor weight (mean 110.7 mg) and vascularization.
- Tumor growth was successfully initiated across different spheroid cell counts.
Conclusions:
- The CAM assay is a feasible model for initiating fibrosarcoma spheroid growth.
- This model holds potential for future research into human fibrosarcoma cell spheroids.
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