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PtrSAUR32 Interacts with PtrPP2C.Ds to Regulate Root Growth in Citrus
Xiaoli Wang1,2, Xiaoya Li1,2, Saihang Zheng1,2
1Citrus Research Institute, Southwest University/Chinese Academy of Agricultural Sciences, Beibei, Chongqing 400712, China.
Plants (Basel, Switzerland)
|June 13, 2025
Summary
Small auxin-up-regulated RNA (SAUR) genes regulate plant growth. Poncirus trifoliata PtrSAUR32 promotes citrus root growth by interacting with protein phosphatases, acting downstream of PtrARF8.
Area of Science:
- Plant Biology
- Molecular Genetics
- Biochemistry
Background:
- Small auxin-up-regulated RNA (SAUR) genes are crucial for plant growth and development.
- The specific roles of SAUR genes in citrus root growth remain largely unexplored.
- Understanding SAUR gene function is key to improving citrus rootstock development.
Purpose of the Study:
- To investigate the function of SAUR genes in Poncirus trifoliata root growth.
- To elucidate the molecular mechanism by which PtrSAUR32 regulates root development.
- To identify key protein interactions involved in PtrSAUR32-mediated root growth.
Main Methods:
- Gene expression analysis of SAUR family members in Poncirus trifoliata roots.
- Overexpression of PtrSAUR32 in citrus to assess its effect on root growth.
- Yeast one-hybrid (Y1H) and dual-luciferase reporter assays to determine transcriptional regulation.
- Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays to investigate protein-protein interactions.
Main Results:
- PtrSAUR32 expression is significantly correlated with primary root length and highly expressed in root tips.
- Overexpression of PtrSAUR32 promotes primary root growth and alters auxin-related gene expression.
- PtrSAUR32 is transcriptionally regulated by PtrARF8.
- PtrSAUR32 interacts with PtrPP2C.D subfamily members (PtrPP2C.D1, PtrPP2C.D6, PtrPP2C.D7), with PtrPP2C.D7 interacting with PtrHA.
Conclusions:
- PtrSAUR32 plays a vital role in regulating citrus root growth, acting downstream of PtrARF8.
- The PtrSAUR32-PtrPP2C.D-PtrHA pathway is a novel mechanism for auxin-mediated root development.
- This study provides insights into the molecular basis of root growth regulation in citrus.
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