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Stress Response System01:21

Stress Response System

The stress response system, also known as the fight-or-flight response, is the body's automatic physiological reaction to perceived threats. Hans Selye introduced the concept of General Adaptation Syndrome (GAS) to describe the predictable pattern of changes that occur in response to stress. GAS consists of three sequential stages: alarm, resistance, and exhaustion. This model helps explain how chronic stress can contribute to health problems.
Alarm stage
In the alarm stage, the body's initial...
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  1. Home
  3. Identification And Analysis Of Stress-associated Protein (sap) Transcription Factor Family Members In Pinus Massoniana.
  1. Home
  3. Identification And Analysis Of Stress-associated Protein (sap) Transcription Factor Family Members In Pinus Massoniana.

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Identification and Analysis of Stress-Associated Protein (SAP) Transcription Factor Family Members in Pinus

Yulu Zhao1,2, Xingyue Ren1,2, Jingjing Zhang1,2

  • 1State Key Laboratory of Tree Genetics and Breeding, Nanjing Forestry University, Nanjing 210037, China.

Plants (Basel, Switzerland)
|June 13, 2025

View abstract on PubMed

Summary
This summary is machine-generated.

This study identified 17 stress-associated protein (SAP) genes in Masson pine, revealing distinct responses to various stresses like ABA and NaCl. These findings enhance understanding of plant stress resistance mechanisms.

Keywords:
Pinus massonianaabiotic stressexpression patternstress-associated proteins (SAPs)

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Area of Science:

  • Plant Molecular Biology
  • Plant Genetics
  • Stress Physiology

Background:

  • Stress-associated proteins (SAPs) are crucial for plant stress responses and belong to the A20/AN1 zinc finger protein family.
  • Research on SAP gene families in Pinus massoniana (Masson pine) is limited, hindering a full understanding of its stress tolerance.
  • Systematic identification and characterization of SAP genes are needed to elucidate their roles in P. massoniana.

Purpose of the Study:

  • To identify and characterize the SAP gene family in Pinus massoniana.
  • To investigate the expression patterns and functional roles of PmSAP genes under various stress conditions.
  • To provide a theoretical basis for understanding stress resistance in P. massoniana.

Main Methods:

  • Identification of 17 PmSAP genes from P. massoniana.
  • Phylogenetic analysis to determine gene relationships and conserved motif identification.
  • Analysis of gene expression using transcriptome data and quantitative real-time PCR (qRT-PCR) under stress treatments (ABA, MeJA, H2O2, ETH, NaCl).
  • Functional validation of transcriptional activity using yeast systems.

    • Main Results:

    • Seventeen PmSAP genes were identified, clustering into five clades with 10 conserved motifs.
    • Differential expression patterns were observed; PmSAP8 and PmSAP12 responded strongly to ABA, MeJA, and H2O2, while PmSAP3 and PmSAP5 were upregulated by ETH and NaCl.
    • Yeast assays confirmed PmSAP6/8/12 as transcriptional activators and PmSAP3 and PmSAP5 as transcriptional repressors.

    Conclusions:

    • The study successfully identified and characterized the PmSAP gene family in P. massoniana.
    • Specific PmSAP genes exhibit distinct responses to various abiotic and hormonal stresses, highlighting their diverse roles.
    • The findings provide valuable insights into the molecular mechanisms underlying stress tolerance in Masson pine.